Identification of drought stress related proteins from 1Sl(1B) chromosome substitution line of wheat variety Chinese Spring

Abstract

Background: Wheat, one of the most important crops, has a detrimental effect on both yield and quality under drought stress. As our preliminary experiment showed that the Chinese Spring wheat-Aegilops longissima chromosome substitution line CS-1S^l (1B) had a better drought tolerance than CS, the substitution line CS-1S^l(1B) was used to identify drought stress related proteins by means of a comparative proteome approach in this work. Our present study aimed to explore the gene resources for drought resistance in 1S^l genome.

Result: Our results showed that drought stress induced downregulation of relative water and chlorophyll contents and the upregulation of proline content, and further influencing grain filling shortening and significant decrease of plant height, B-type starch granule numbers, grain number and weight. In total, 25 grain albumin and globulin protein spots were found to be specifically encoded by the 1S^l chromosome. In addition, 17 protein spots respected 13 unique proteins were identified by MALDI-TOF/TOF MS, which were mainly involved in adverse defense and gluten quality. Among them, ascorbate peroxidase, serpin-Z2B and alpha-amylase/trypsin inhibitor were upregulated under drought stress. These proteins play important roles in plant drought defenses through various metabolic pathways.

Conclusion: Our results indicate that the 1S^l chromosome of Aegilops longissima has potential gene resources that could be useful for improving wheat drought resistance.

Keywords: 2-DE; Aegilops longissima 1Sl chromosome; Drought tolerance; Proteome; Wheat.

Fig. 1

Soil water content changes and leaf physiological parameter changes. CK represents the control group, and DS represents the drought stress treated group. a Soil water content changes; b relative water content; c proline content; d chlorophyll content; * and**indicate a significant difference at p < 0.05 and p < 0.01 level by t test, respectively

Fig. 2

Grain morphology and SEM observation of CS-1S^l (1B) under well-watered and drought stress conditions. The scale is shown at the bottom right corner of the Figure, and one space is 5 μm, total is 50 μm

Fig. 3

Functional distribution of 17 seed proteins encoded by 1S^l genome from CS-1S^l (1B)

Fig. 4

Proteome maps of CS-1S¹ (1B) mature grains under well-watered (CK) and drought stress (DS) conditions. Sots S1 to S25 were the 1S¹-encoded protein