Regulation by calcium of hormone-insensitive adenylate cyclase and calmodulin-dependent guanylate cyclase in Tetrahymena plasma membrane

Abstract

Agents such as 5'-guanylyl-imidodiphosphate(GppNHp), fluoride and forskolin did not activate adenylate cyclase from Tetrahymena. In addition, the cyclase was not stimulated by hormones including catecholamines and glucagon when assayed with or without GppNHp at conditions where they increased adenylate cyclase activity from rat heart. Sodium azide, NaNO2 or N-methyl-N'-nitro-N-nitroguanidine (MNNG) failed to activate Tetrahymena guanylate cyclase. Adenylate cyclase activity was activated at low free Ca2+ level and inhibited at high levels, while guanylate cyclase activity was activated by Tetrahymena calmodulin only at high physiological concn of Ca2+.