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. 1975;20(6):480-7.
doi: 10.1007/BF02891707.

Transport properties of membrane vesicles from Acholeplasma laidlawii. II. Kinetic characteristics and specificity of glucose transport system

L F Panchenko, N S Fedotov, M A Tarshis

Transport properties of membrane vesicles from Acholeplasma laidlawii. II. Kinetic characteristics and specificity of glucose transport system

L F Panchenko et al. Folia Microbiol (Praha). 1975.

Abstract

The glucose transport system of membrane vesicles isolated from Acholeplasma laidlawii is saturable, with a Km of 21.2 mum and V of 0.68 nmol min-1 (mg protein)-1. The process is pH-dependent and a break occurs in the Arrhenius plot at 15 degrees C. Exogenous substrates did not stimulate glucose transport probably due to their inability to penetrate into membrane vesicles. 3-O-Methylglucose and 6-deoxyglucose competitively inhibited glucose transport. Maltose inhibited transport of glucose noncompetitively. These sugars also elicited glucose efflux from preloaded membrane vesicles.

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