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. 2017 May 12:18:38-46.
doi: 10.1016/j.nmni.2017.05.003. eCollection 2017 Jul.

Dakarella massiliensis gen. nov., sp. nov., strain ND3T: a new bacterial genus isolated from the female genital tract

N Dione  1 , J Rathored  1 , E Tomei  1 , J-C Lagier  1 , S Khelaifia  1 , C Robert  1 , F Bretelle  1   2 , D Raoult  1   3 , P-E Fournier  1 , F Fenollar  1
Affiliations

Dakarella massiliensis gen. nov., sp. nov., strain ND3T: a new bacterial genus isolated from the female genital tract

N Dione et al. New Microbes New Infect. .

Abstract

Strain ND3T was isolated from the genital tract of a 28-year-old woman with bacterial vaginosis. This strain exhibited a 16S rRNA gene sequence similarity of 92.4% with Sutterella wadsworthensis, the phylogenetically closest species with standing in nomenclature. Strain ND3T was a strictly anaerobic Gram-negative rod and member of the family Sutterellaceae. It exhibited a genome of 2 476 884 bp containing 2175 protein-coding and 62 RNA genes. On the basis of these data, we propose the creation of 'Dakarella massiliensis' sp. nov. with strain ND3T (= CSUR P1938 = DSM 100447) as the type strain.

Keywords: bacterial vaginosis; culturomics; taxono-genomics.

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Figures

Fig. 1
Fig. 1
Phylogenetic tree highlighting position of ‘Dakarella massiliensis’ strain ND3T relative to other type strains within Sutterellaceae family. Sequences were aligned using CLUSTALW, and phylogenetic inferences were obtained using maximum-likelihood method within MEGA software. Numbers at nodes are percentages of bootstrap values obtained by repeating analysis 500 times to generate majority consensus tree. GenBank accession numbers are indicated in tree. Lautropia mirabilis was used as outgroup. Scale bar represents 2% nucleotide sequence divergence.
Fig. 2
Fig. 2
Reference mass spectrum from ‘Dakarella massiliensis’ strain ND3T. Spectra from 12 individual colonies were compared and reference spectrum generated.
Fig. 3
Fig. 3
Transmission electron microscopy of ‘Dakarella massiliensis’ strain ND3T using Tecnai G20(Fei) at operating voltage of 60 kV. Scale bar = 500 nm.
Fig. 4
Fig. 4
Gram staining of ‘Dakarella massiliensis’ strain ND3T.
Fig. 5
Fig. 5
Graphical circular map of chromosome. From outside to centre: genes on forward strand coloured by COGs categories (only genes assigned to COGs), genes on reverse strand coloured by COGs categories (only genes assigned to COGs), RNA genes (tRNAs green, rRNAs red), GC content and GC skew. COGS, Clusters of Orthologous Groups database.
See this image and copyright information in PMC

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