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Editorial
. 2017 May 9:4:36.
doi: 10.21037/sci.2017.04.06. eCollection 2017.

Exploiting induced senescence in intestinal organoids to drive enteroendocrine cell expansion

Robert G Ramsay  1 , Helen E Abud  2
Affiliations
Editorial

Exploiting induced senescence in intestinal organoids to drive enteroendocrine cell expansion

Robert G Ramsay et al. Stem Cell Investig. .
No abstract available

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Conflict of interest statement

Conflicts of Interest: The authors have no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Intestinal organoids are readily generated from the small intestine in the presence of EGFR, NOTCH and WNT stimuli and have the capacity to generate multiple cell lineages. These cell lineages arise from a stem cell population identified by robust expression of R-spondin receptor, Lgr5. A, when EGFR signalling is blocked (EGFRi) cells withdraw from the cell cycle. Under these conditions expression of the enteroendocrine lineage marker chromogranin A (ChgA) is increased slightly and is increased further by subsequent withdrawal of NOTCH and WNT stimuli. B, under these conditions of growth factor blockade an expanded proportion of quiescent Lgr5+ve cells populate the smaller organoids. These however retain multi-lineage potential as well as a source of enriched heterogeneous enteroendocrine cells.
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References

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