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. 2017 Jul;46(6):782-788.
doi: 10.1097/MPA.0000000000000847.

Exosomal Markers (CD63 and CD9) Expression Pattern Using Immunohistochemistry in Resected Malignant and Nonmalignant Pancreatic Specimens

Moh'd Khushman  1 , Arun Bhardwaj, Girijesh Kumar Patel, Javier Ariel Laurini, Kelly Roveda, Marcus C Tan, Mary C Patton, Seema Singh, William Taylor, Ajay P Singh
Affiliations

Exosomal Markers (CD63 and CD9) Expression Pattern Using Immunohistochemistry in Resected Malignant and Nonmalignant Pancreatic Specimens

Moh'd Khushman et al. Pancreas. 2017 Jul.

Abstract

Objectives: Exosomes are important mediators in intercellular communications and play a role in cancer progression and metastasis. Exosomal membranes are enriched in endosome-specific tetraspanins (CD9 and CD63). Here, we explored the expression of CD63 and CD9 utilizing immunohistochemistry in malignant and nonmalignant cells in 29 resected pancreatic specimens (RPSs) of mixed racial background.

Methods: The pathologic tissues (PTs) and adjacent normal tissues (ANTs) in each RPS were stained for CD63 and CD9. Two pathologists independently scored the expression of CD63 and CD9. Staining intensity was graded from 1 to 3. Staining percentage was estimated in 10% increments. An average Quick score (Q score) (intensity × percentage of staining) was calculated. Unpaired t test was used for statistical analysis.

Results: The mean multiplicative Q score for CD63 and CD9 expression is higher in PTs (209 and 72) compared with ANTs (154 and 24) (P = 0.0041, P = 0.0018), respectively. The Mean Q score for CD63 and CD9 expression is higher in the malignant PTs (231 and 85) compared with ANTs (129 and 25) (P < 0.0001 and P < 0.0124).

Conclusions: Exosomal markers (CD63 and CD9) expression assessment using immunohistochemistry is feasible in RPS. The expression of CD63 and CD9 is higher in PTs and malignant PTs compared with their ANTs.

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Conflict of interest statement

Disclosure: The authors of this manuscript have no conflicts of interest to disclose.

Figures

Figure 1
Figure 1
H&E staining and CD63 and CD9 expression in malignant pathologic tissues (PTs) and their adjacent normal tissues (ANTs). (200X)
Figure 2
Figure 2
H&E staining and CD63 and CD9 expression in premalignant pathologic tissues and their adjacent normal tissues (ANTs). (200X)
Figure 3
Figure 3
H&E staining and CD63 and CD9 expression in inflammatory pathologic tissues and their adjacent normal tissues (ANTs). 200X
Figure 4
Figure 4
Exosomal markers CD9 and CD63 expression pattern in resected pancreatic tissues. Pathologic Tissues (PTs); Adjacent Normal Tissues (ANTs); Malignant PTs (MPTs); Malignant ANTs (MANTs); Premalignant PTs (PPTs); Premalignant ANTs (PANTs); Inflammatory PTs (IPTs); Inflammatory ANTs (IANTs); ***P <0.001; **P <0.01; *P <0.05.
See this image and copyright information in PMC

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