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Review
. 2017 Jun;57(2):139-147.
doi: 10.1007/s12088-017-0650-8. Epub 2017 Apr 25.

Gene Specific DNA Sensors for Diagnosis of Pathogenic Infections

Manali Datta  1 , Dignya Desai  1 , Ashok Kumar  2
Affiliations
Review

Gene Specific DNA Sensors for Diagnosis of Pathogenic Infections

Manali Datta et al. Indian J Microbiol. 2017 Jun.

Abstract

Gene specific DNA based sensors have potential applications for rapid and real time monitoring of hybridization signal with the target nucleic acid of pathogens. Different types of DNA based sensors and their applications have been studied for rapid and accurate detection of pathogens causing human diseases. These sensors are based on surface plasmon resonance, quantum-dots, molecular beacons, piezoelectric and electrochemical etc. Curbing epidemics at an early stage is one of the massive challenges in healthcare systems. Timely detection of the causative organism may provide a solution to restrain mortality caused by the disease. With the advent of interdisciplinary sciences, bioelectronics has emerged as an effective alternative for disease diagnostics. Gene specific DNA sensors present themselves as cost-effective, sensitive and specific platforms for detection of disease causing pathogens. The mini review explores different transducer based sensors and their potential in diagnosis of acute and chronic diseases.

Keywords: DNA sensors; E. coli; M. tuberculosis; N. meningitidis; Pathogens; S. pyogenes; S. typhi.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Process flow of biosensing mechanism
Fig. 2
Fig. 2
Schematic representation of SPR for detection of target ssDNA
Fig. 3
Fig. 3
Schematic representation of FRET based DNA sensor
Fig. 4
Fig. 4
Schematic diagram of quantum-dots based DNA sensor
Fig. 5
Fig. 5
Functioning of MB sensor and subsequent fluorescent signal on excitation
Fig. 6
Fig. 6
Piezoelectric based DNA sensor for detection of pathogen
Fig. 7
Fig. 7
Schematic presentation of electrochemical DNA sensor
See this image and copyright information in PMC

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