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Comparative Study
. 2017 Jun;96(24):e7139.
doi: 10.1097/MD.0000000000007139.

The analyzation and clinical evaluation of ECLIA and CMIA in the detection of Treponema pallidum

Jiansuo Zhou  1 , Yongming Liang, Jie Zhang, Liyan Cui
Affiliations
Comparative Study

The analyzation and clinical evaluation of ECLIA and CMIA in the detection of Treponema pallidum

Jiansuo Zhou et al. Medicine (Baltimore). 2017 Jun.

Abstract

To evaluate the clinical application value of electrochemiluminescence immunoassay analyzer (ECLIA) and chemiluminescent magnetic microparticle immunoassay (CMIA) in the detection of Treponema pallidum (TP).A total of 1225 patients in Peking University Third Hospital was enrolled from June 2014 to October 2014. ECLIA and CMIA were applied to detect the serum anti-TP. The positive rate was analyzed. RIBA was taken as a golden standard to evaluate the sensitivity, the specificity, the positive predictive value, the negative predictive value, and the accuracy of ECLIA and CMIA. A correlation analysis between 2 assays was conducted, and that between assay and RIBA. We also evaluate the clinical value of TPPA in the detection of T pallidum.The positive rate of CMIA and ECLIA is 10.63% and 9.89%, respectively, showing no statistically significant difference (P > .05). For CMIA, ECLIA, and TPPA, the sensitivity is 99.16%, 99.16%, and 99.16%, the specificity is 98.99%, 99.82%, and 100%, the positive predictive value is 91.47%, 98.33%, and 100%, the negative predictive value is 99.91%, 99.91%, and 99.91%, the coincidence rate is 99.01% (Kappa = 0.895), 99.75% (Kappa = 0.997), and 99.92% (Kappa = 0.998), respectively.The result shows high correlation between ECLIA and CMIA. Both have high sensitivity and specificity and can be used as screening tests for the diagnosis of T pallidum in common condition.

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Conflict of interest statement

The authors have no conflicts of interest to disclose.

Figures

Figure 1
Figure 1
Comparison of the result of the CMIA and ECLIA in special groups.
See this image and copyright information in PMC

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References

    1. The Chinese Center for Disease Control and Prevention, Public Health Surveillance and Information Service Center. The Chinese A, B and C class infectious diseases epidemic dynamic introduction in December 2007. J Dis Surveil 2008;23:4.
    1. The Ministry of Health of the Chinese Syphilis Prevention and Control Planning (2010–2020). 2010. Available at: http://www.moh.gov.cn/mohbgt/s10788/201006/47778.shtml.
    1. The Ministry of Health of the 2012 Annual National Statutory Epidemic Situation of Infectious Diseases, Available at: http://www.nhfpc.gov.cn/jkj/s3578/201304/b540269c8e5141e6bb2d00ca539bb9f....
    1. Meng Z. Syphilis detection technology progress. J Clin Lab Med 2011;8:2627–8.
    1. Mo X, Jin Y, Yang Y, et al. Evaluation of a new chemiluminescence immunoassay for diagnosis of syphilis. Eur J Med Res 2010;15:66–9. - PMC - PubMed