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Review
. 2017 Jun 14;9(1):56.
doi: 10.1186/s13073-017-0448-7.

Dissecting the human microbiome with single-cell genomics

Andrew C Tolonen  1 , Ramnik J Xavier  2   3   4   5
Affiliations
Review

Dissecting the human microbiome with single-cell genomics

Andrew C Tolonen et al. Genome Med. .

Abstract

Recent advances in genome sequencing of single microbial cells enable the assignment of functional roles to members of the human microbiome that cannot currently be cultured. This approach can reveal the genomic basis of phenotypic variation between closely related strains and can be applied to the targeted study of immunogenic bacteria in disease.

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Figures

Fig. 1
Fig. 1
Overview of steps for single-cell sequencing of microbial genomes. Experimental steps include (upper half) isolation and lysis of single cells with subsequent amplification of their genomes, followed by (lower half) high-throughput sequencing, genome assembly and genome analysis. FACS fluorescence-activated cell sorting, MDA multiple displacement amplification, PCR polymerase chain reaction
See this image and copyright information in PMC

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