doi: 10.1007/BF00274019.
A restriction endonuclease cleavage map of bacteriophage P2
- PMID: 286153
- DOI: 10.1007/BF00274019
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A restriction endonuclease cleavage map of bacteriophage P2
Mol Gen Genet.
.
Abstract
A restriction endonuclease cleavage map of phage P2 was constructed. The enzymes used and, within parenthesis, the number of their cleavage sites on the P2 lg cc DNA molecule were: AvaI(3), BalI(1), BAMI(3), BglII(3), HaeIII (more than 40; only three were mapped), HindIII(0), HpaI(10), KpnI(3), PstI(3), SalI(2) and SmaI(2). The EcoRI cleavage sites (3), as determined earlier, were used as reference points for this study. The DNAs of a variety of P2 mutants carrying chromosomal aberrations (dell, del2, del3, del6, vir22, vir37(2), vir79 and vir94) were also similarly examined.
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