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. 1985 Apr;16(3-4):170-2.
doi: 10.1007/BF01983130.

The use of an in vitro binding assay to predict histamine H2-antagonist activity

The use of an in vitro binding assay to predict histamine H2-antagonist activity

D B Norris et al. Agents Actions. 1985 Apr.

Abstract

Thirty-seven compounds were evaluated for their ability to inhibit histamine stimulated adenylate cyclase and to inhibit 3H-tiotidine binding. The compounds examined included a number of known H2-antagonists and a number of potential H2-antagonists of diverse chemical structure. The correlation between the Ki values from the binding assay and from the inhibition of adenylate cyclase was calculated to be r = 0.99, p less than 0.001. Thus 3H-tiotidine binding in guinea-pig cerebral cortex can be used to give a valid assessment of histamine H2-receptor activity.

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References

    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Nature. 1976 Mar 11;260(5547):163-5 - PubMed
    1. Biochem J. 1971 Feb;121(3):561-2 - PubMed
    1. Nature. 1983 Jul 7-13;304(5921):65-7 - PubMed

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