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Multicenter Study
. 2017 Nov;89(11):2047-2050.
doi: 10.1002/jmv.24874. Epub 2017 Jul 6.

Interlaboratory quality control of total HIV-1 DNA load measurement for multicenter reservoir studies

Affiliations
Multicenter Study

Interlaboratory quality control of total HIV-1 DNA load measurement for multicenter reservoir studies

Pierre Gantner et al. J Med Virol. 2017 Nov.

Abstract

Background: Viral reservoirs represent an important barrier to HIV cure. Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter-laboratory reproducibility of total HIV-1-DNA quantification.

Methods: Ten laboratories of the ANRS-AC11 working group participated by quantifying HIV-DNA with a real-time qPCR assay (Biocentric) in four samples (QCMD).

Results: Good reproducibility was found between laboratories (standard deviation ≤ 0.2 log10 copies/106 PBMC) for the three positive QC that were correctly classified by each laboratory (QC1<QC2<QC3).

Conclusions: Results of this QC validate the feasibility of multicenter studies using this standardized assay.

Keywords: HIV-1 DNA; interlaboratory quality control; quantification.

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