Interlaboratory quality control of total HIV-1 DNA load measurement for multicenter reservoir studies
- PMID: 28617961
- DOI: 10.1002/jmv.24874
Interlaboratory quality control of total HIV-1 DNA load measurement for multicenter reservoir studies
Abstract
Background: Viral reservoirs represent an important barrier to HIV cure. Accurate markers of HIV reservoirs are needed to develop multicenter studies. The aim of this multicenter quality control (QC) was to evaluate the inter-laboratory reproducibility of total HIV-1-DNA quantification.
Methods: Ten laboratories of the ANRS-AC11 working group participated by quantifying HIV-DNA with a real-time qPCR assay (Biocentric) in four samples (QCMD).
Results: Good reproducibility was found between laboratories (standard deviation ≤ 0.2 log10 copies/106 PBMC) for the three positive QC that were correctly classified by each laboratory (QC1<QC2<QC3).
Conclusions: Results of this QC validate the feasibility of multicenter studies using this standardized assay.
Keywords: HIV-1 DNA; interlaboratory quality control; quantification.
© 2017 Wiley Periodicals, Inc.
Similar articles
-
Blinded, multicenter quality control study for the quantification of human immunodeficiency virus type 1 RNA in plasma by the Belgian AIDS reference laboratories.Clin Microbiol Infect. 2000 Apr;6(4):213-7. doi: 10.1046/j.1469-0691.2000.00048.x. Clin Microbiol Infect. 2000. PMID: 11168110
-
Quantification of viral DNA during HIV-1 infection: A review of relevant clinical uses and laboratory methods.Pathol Biol (Paris). 2015 Feb;63(1):53-9. doi: 10.1016/j.patbio.2014.07.007. Epub 2014 Sep 8. Pathol Biol (Paris). 2015. PMID: 25201144 Review.
-
[Analysis of the results of the SEIMC External Quality Control Program for HIV-1 and HCV viral loads, 2007].Enferm Infecc Microbiol Clin. 2008 Nov;26 Suppl 13:8-13. Enferm Infecc Microbiol Clin. 2008. PMID: 19100161 Spanish.
-
[Analysis of the results of the SEIMC External Quality Control Program for HIV-1 and HCV viral loads. Year 2008].Enferm Infecc Microbiol Clin. 2010 Jan;28 Suppl 1:7-11. doi: 10.1016/S0213-005X(10)70002-1. Enferm Infecc Microbiol Clin. 2010. PMID: 20172417 Spanish.
-
Total HIV-1 DNA, a Marker of Viral Reservoir Dynamics with Clinical Implications.Clin Microbiol Rev. 2016 Oct;29(4):859-80. doi: 10.1128/CMR.00015-16. Clin Microbiol Rev. 2016. PMID: 27559075 Free PMC article. Review.
Cited by
-
External quality assessment of HIV-1 DNA quantification assays used in the clinical setting in Italy.Sci Rep. 2022 Feb 28;12(1):3291. doi: 10.1038/s41598-022-07196-2. Sci Rep. 2022. PMID: 35228581 Free PMC article.
-
Impact of Different Antiretroviral Strategies on Total HIV-DNA Level in Virologically Suppressed HIV-1 Infected Patients.Curr HIV Res. 2017;15(6):448-455. doi: 10.2174/1570162X16666171206121026. Curr HIV Res. 2017. PMID: 29210661 Free PMC article.
-
Clinical Relevance of Total HIV DNA in Peripheral Blood Mononuclear Cell Compartments as a Biomarker of HIV-Associated Neurocognitive Disorders (HAND).Viruses. 2017 Oct 31;9(11):324. doi: 10.3390/v9110324. Viruses. 2017. PMID: 29088095 Free PMC article. Review.
-
Total HIV DNA: a global marker of HIV persistence.Retrovirology. 2018 Apr 3;15(1):30. doi: 10.1186/s12977-018-0412-7. Retrovirology. 2018. PMID: 29615133 Free PMC article. Review.
-
In-depth validation of total HIV-1 DNA assays for quantification of various HIV-1 subtypes.Sci Rep. 2018 Nov 22;8(1):17274. doi: 10.1038/s41598-018-35403-6. Sci Rep. 2018. PMID: 30467426 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
