Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Mar;32(3):e22281.
doi: 10.1002/jcla.22281. Epub 2017 Jun 15.

Reduced expression of circRNA hsa_circ_0003159 in gastric cancer and its clinical significance

Mengqian Tian  1 , Ruoyu Chen  1 , Tianwen Li  1 , Bingxiu Xiao  1
Affiliations

Reduced expression of circRNA hsa_circ_0003159 in gastric cancer and its clinical significance

Mengqian Tian et al. J Clin Lab Anal. 2018 Mar.

Abstract

Background: Circular RNAs (circRNAs) play a crucial role in the occurrence of several diseases including cancers. However, little is known about circRNAs' diagnostic values for gastric cancer, one of the worldwide most common diseases of mortality.

Methods: The hsa_circ_0003159 levels in 108 paired gastric cancer tissues and adjacent non-tumorous tissues from surgical patients with gastric cancer were first detected by real-time quantitative reverse transcription-polymerase chain reaction. Then, the relationships between hsa_circ_0003159 expression levels in gastric cancer tissues and the clinicopathological factors of patients with gastric cancer were analyzed. Finally, its diagnostic value was evaluated through the receiver operating characteristic curve.

Results: Compared with paired adjacent non-tumorous tissues, hsa_circ_0003159 expression was significantly down-regulated in gastric cancer tissues. What is more, we found that hsa_circ_0003159 expression levels were significantly negatively associated with gender, distal metastasis, and tumor-node-metastasis stage.

Conclusions: All of the results suggest that hsa_circ_0003159 may be a potential cancer marker of patients with gastric cancer.

Keywords: biomarker; circular RNA; gastric cancer; gene diagnosis; hsa_circ_0003159.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The sequencing result of the products of qRT‐PCR for hsa_circ_0003159
Figure 2
Figure 2
The expression levels of hsa_circ_0003159 in gastric cancer samples. Hsa_circ_0003159 and GAPDH expression levels were detected by qRT‐PCR. The ΔC t method was used to analyze the relative expression of hsa_circ_0003159. n=108, P˂.001
Figure 3
Figure 3
The ROC curve of hsa_circ_0003159
See this image and copyright information in PMC

References

    1. Yao T, Chen Q, Fu L, Guo J. Circular RNAs: biogenesis, properties, roles, and their relationships with liver diseases. Hepatol Res. 2017;47:497‐504. - PubMed
    1. Chen S, Li T, Zhao Q, Xiao B, Guo J. Using circular RNA hsa_circ_0000190 as a new biomarker in the diagnosis of gastric cancer. Clin Chim Acta. 2017;466:167‐171. - PubMed
    1. Li P, Chen H, Chen S, et al. Circular RNA 0000096 affects cell growth and migration in gastric cancer. Br J Cancer. 2017;116:626‐633. - PMC - PubMed
    1. Li P, Chen S, Chen H, et al. Using circular RNA as a novel type of biomarker in the screening of gastric cancer. Clin Chim Acta. 2015;444:132‐136. - PubMed
    1. Fu L, Yao T, Chen Q, Mo X, Hu Y, Guo J. Screening differential circular RNA expression profiles reveals hsa_circ_0004018 is associated with hepatocellular carcinoma. Oncotarget. 2017. 10.18632/oncotarget.16881. - DOI - PMC - PubMed