Differential expression of long noncoding RNAs in congenital microtia

Abstract

Objective: To analyse lncRNA expression profiles in microtia using bioinformatics analysis.

Methods: We examined lncRNA expression profiles in residual ear cartilage and normal ear cartilage from individual congenital microtia patients.

Results: The gene chips used in this study included 30586 lncRNAs and 26109 mRNA probes. Intotal, 180 lncRNAs with differential expression weredetected in the residual ear cartilage compared with the normal cartilage, including 74 up-regulated and 106down-regulated lncRNAs. Signalling pathway analysis highlighted glyceride metabolism, osteoclast differentiation, andtumour growth. The results of qRT-PCR analysis were consistent with those of themicroarray.

Conclusion: Differential expression of lncRNAs occurs in microtia. These lncRNAs and related signalling pathways may play an important role in the occurrence and development ofmicrotia.

Keywords: Microarray; Microtia; lncRNAs.