The effects of alpha- and beta-adrenergic stimulation on contractility and prostaglandin (prostaglandins E2 and F2 alpha and 6-keto-prostaglandin F1 alpha) production of pregnant human myometrial strips

Abstract

The effects of catecholamines and alpha- and beta-adrenergic agonists and antagonists on the spontaneous contractility of superfused pregnant human myometrial strips are reported. Prostaglandins (prostaglandins E2 and F2 alpha and 6-keto-prostaglandin F1 alpha) were analyzed in the effluent of the superfusion medium by specific radioimmunoassays. Both epinephrine and norepinephrine dose-dependently (10 ng/ml to 1 microgram/ml) stimulated the contractility of the myometrial strips and significantly increased the synthesis of all prostaglandins assayed. alpha-Adrenergic blockers inhibited the catecholamine-induced increase in contractility. This was associated with decreased prostaglandins F2 alpha and E2 concentrations and a further increase in 6-keto-prostaglandin F1 alpha levels. Exclusive beta-adrenergic stimulation with beta-mimetic drugs had the same effect. Conversely, epinephrine stimulation together with beta-blockers resulted in a further increase in the prostaglandins F2 alpha and E2 release of the myometrial strips. This effect was even more pronounced with specific alpha-adrenergic stimulant drugs. Our results demonstrate the interrelationship of alpha- and beta-adrenergic stimulation and the prostaglandin system. alpha-Adrenergic stimulation increases myometrial contractility and the synthesis of prostaglandins F2 alpha and E2. beta-Adrenergic stimulation reduces contractility by further enhancing 6-keto-prostaglandin F1 alpha production.