Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Mar;76(3):1382-4.
doi: 10.1073/pnas.76.3.1382.

Measurement and purification of human chromosomes by flow cytometry and sorting

A V Carrano, J W Gray, R G Langlois, K J Burkhart-Schultz, M A Van Dilla

Measurement and purification of human chromosomes by flow cytometry and sorting

A V Carrano et al. Proc Natl Acad Sci U S A. 1979 Mar.

Abstract

The 24 human chromosome types of normal diploid fibroblast cell strain were classified into 15 groups by high-resolution flow cytometry on the basis of 33258 Hoechst fluorescence. Chromosomes associated with each group were flow sorted onto microscope slides and identified by quinacrine banding analysis. DNA cytophotometry of metaphase chromosomes from the same cell strain supported and extended this identification. Four of the groups purified were due to chromosomes of a single type--namely, chromosomes 5, 6, 13, and 17. Eight additional groups were also separated and found to contain the following chromosomes: 1 and 2; 3 and 4; 7, 8, and X; 9--12; 14 and 15; 16 and 18; 20 and Y; and 19, 21, and 22. The average purity for the 12 sorted fractions was 78%.

PubMed Disclaimer

References

    1. Chromosoma. 1976 Jul 8;56(3):199-211 - PubMed
    1. Cell. 1977 Apr;10(4):649-57 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3937-41 - PubMed
    1. Somatic Cell Genet. 1977 May;3(3):281-93 - PubMed
    1. In Vitro. 1976 Nov;12(11):777-86 - PubMed

Publication types

LinkOut - more resources