Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Dec;8(6):578-584.
doi: 10.1007/s12975-017-0545-3. Epub 2017 Jun 21.

CCL11 (Eotaxin-1) Levels Predict Long-Term Functional Outcomes in Patients Following Ischemic Stroke

Meaghan Roy-O'Reilly  1 , Rodney M Ritzel  2 , Sarah E Conway  3   4 , Ilene Staff  4 , Gilbert Fortunato  4 , Louise D McCullough  5   6
Affiliations

CCL11 (Eotaxin-1) Levels Predict Long-Term Functional Outcomes in Patients Following Ischemic Stroke

Meaghan Roy-O'Reilly et al. Transl Stroke Res. 2017 Dec.

Abstract

Circulating levels of the pro-inflammatory cytokine C-C motif chemokine 11 (CCL11, also known as eotaxin-1) are increased in several animal models of neuroinflammation, including traumatic brain injury and Alzheimer's disease. Increased levels of CCL11 have also been linked to decreased neurogenesis in mice. We hypothesized that circulating CCL11 levels would increase following ischemic stroke in mice and humans, and that higher CCL11 levels would correlate with poor long-term recovery in patients. As predicted, circulating levels of CCL11 in both young and aged mice increased significantly 24 h after experimental stroke. However, ischemic stroke patients showed decreased CCL11 levels compared to controls 24 h after stroke. Interestingly, lower post-stroke CCL11 levels were predictive of increased stroke severity and independently predictive of poorer functional outcomes in patients 12 months after ischemic stroke. These results illustrate important differences in the peripheral inflammatory response to ischemic stroke between mice and human patients. In addition, it suggests CCL11 as a candidate biomarker for the prediction of acute and long-term functional outcomes in ischemic stroke patients.

Keywords: CCLL1; Eotaxin; Inflammation; Ischemia; Neuroinflammation; Stroke.

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest: The authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
Peripheral CCL11 levels 24 hrs in serum after sham or stroke MCAO surgery in young and aged mice. N=3–4/group. Error bars show Standard Error of the Mean (SEM). **=p<0.01.
Fig 2
Fig 2
Peripheral CCL11 levels 24 hours after the onset of ischemic stroke (n=133) in human patients compared to levels in asymptomatic control serum (n=17). Error bars show SEM. * = p<.05.
Figure 3
Figure 3
A. Correlation between serum concentrations of CCL11 at 24 hours post-stroke and admission NIH stroke severity from ischemic stroke patients as measured by Multiplex Assay. NIHSS was assessed as a continuous variable by non-parametric correlation analysis. B. Correlation between serum concentrations of CCL11 at 24 hours post-stroke and discharge NIH stroke severity in ischemic stroke patients.
See this image and copyright information in PMC

References

    1. Rothenberg ME, Maclean JA, Pearlman E, Luster AD, Leder Pa. Targeted disruption of the chemokine eotaxin partially reduces antigen-induced tissue eosinophilia. J Exp Med. 1997;185(4):785–90. - PMC - PubMed
    1. Emanuele E, Falcone C, D’angelo A. Association of plasma eotaxin levels with the presence and extent of angiographic coronary artery disease. Atherosclerosis. 2006;186(1):140–5. - PubMed
    1. Mir A, Minguez M, Tatay J, et al. Elevated serum eotaxin levels in patients with inflammatory bowel disease. Am J Gastroenterol. 2002;97(6):1452–7. - PubMed
    1. Fryer AD, Stein LH, Nie Z, et al. Neuronal eotaxin and the effects of CCR3 antagonist on airway hyperreactivity and M2 receptor dysfunction. J Clin Invest. 2006;116(1):228–36. - PMC - PubMed
    1. Adar T, Shteingart S, Ben ya’acov A, Bar-gil shitrit A, Goldin E. From airway inflammation to inflammatory bowel disease: eotaxin-1, a key regulator of intestinal inflammation. Clin Immunol. 2014;153(1):199–208. - PubMed

Publication types

LinkOut - more resources