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. 2017 Jun 22;12(6):e0179715.
doi: 10.1371/journal.pone.0179715. eCollection 2017.

Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population

Tefera Tolera Angessa  1 , Xiao-Qi Zhang  1 , Gaofeng Zhou  1 , Sue Broughton  2 , Wenying Zhang  3 , Chengdao Li  1   2
Affiliations

Early growth stages salinity stress tolerance in CM72 x Gairdner doubled haploid barley population

Tefera Tolera Angessa et al. PLoS One. .

Abstract

A doubled haploid (DH) population of barley (Hordeum vulgare L.) generated from salinity tolerant genotype CM72 and salinity sensitive variety Gairdner was studied for salinity stress tolerance at germination, seedling emergence and first leaf full expansion growth stages. Germination study was conducted with deionized water, 150 mM and 300 mM NaCl treatments. Seedling stage salinity tolerance was conducted with three treatments: control, 150 mM NaCl added at seedling emergence and first leaf full expansion growth stages. Results from this study revealed transgressive phenotypic segregations for germination percentage and biomass at seedling stage. Twelve QTL were identified on chromosomes 2H-6H each explaining 10-25% of the phenotypic variations. A QTL located at 176.5 cM on chromosome 3H was linked with fresh weight per plant and dry weight per plant in salinity stress induced at first leaf full expansion growth stage, and dry weight per plant in salinity stress induced at seedling emergence. A stable QTL for germination at both 150 and 300 mM salinity stress was mapped on chromosome 2H but distantly located from a QTL linked with seedling stage salinity stress tolerance. QTL, associated markers and genotypes identified in this study play important roles in developing salinity stress tolerant barley varieties.

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Conflict of interest statement

Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial competing interests.

Figures

Fig 1
Fig 1. Germination stage salinity stress effect on barley CM72 x Gairdner doubled haploid population.
Ratio of germination percentage (A) in 150 mM NaCl treatment to deionized water treatment, and (B) in 300 mM NaCl treatment to deionized water treatment.
Fig 2
Fig 2. Seedling stages salinity stress effect on barley CM72 x Gairdner doubled haploid population.
Fresh weight per plant (A & B); dry weight per plant (C & D)ratio in salinity stress treatments to that in control treatment and expressed in percentage.
Fig 3
Fig 3. Salinity stress tolerance linked QTL identified in CM72 x Gairdner doubled haploid population.
QTL linked with germination percentage in NaCl treatments of 150 mM (GP150), 300 mM (GP300a,GP300b); fresh weight per plant in control treatment (FWct), fresh weight per plant in 150 mM NaCl treatments at seedling emergence (FWem1, FWem2, FWem3), and fresh weight per plant at first leaf expansion (FWex1, FWex2); dry weight per plant in control treatment (DWct), dry weight per plant in 150 mM NaCl treatments at seedling emergence (DWem1, DWem2), and dry weight per plant at first leaf expansion (DWex1, DWex2)in a hydroponic study; and QTL linked with plant height at maturity (Ht1, Ht2).
See this image and copyright information in PMC

References

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