High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates

Kaoru Seiriki¹, Atsushi Kasai², Takeshi Hashimoto³, Wiebke Schulze⁴, Misaki Niu⁴, Shun Yamaguchi⁵, Takanobu Nakazawa⁶, Ken-Ichi Inoue⁷, Shiori Uezono⁷, Masahiko Takada⁷, Yuichiro Naka⁴, Hisato Igarashi⁴, Masato Tanuma⁴, James A Waschek⁸, Yukio Ago⁹, Kenji F Tanaka¹⁰, Atsuko Hayata-Takano¹¹, Kazuki Nagayasu⁴, Norihito Shintani⁴, Ryota Hashimoto¹², Yasuto Kunii¹³, Mizuki Hino¹⁴, Junya Matsumoto¹⁴, Hirooki Yabe¹⁴, Takeharu Nagai¹⁵, Katsumasa Fujita¹⁶, Toshio Matsuda⁴, Kazuhiro Takuma¹⁷, Akemichi Baba¹⁸, Hitoshi Hashimoto¹⁹

Affiliations

¹ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan; Interdisciplinary Program for Biomedical Sciences, Institute for Academic Initiatives, Osaka University, Suita, Osaka 565-0871, Japan.
² Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: kasai@phs.osaka-u.ac.jp.
³ Department of Electrical & Electronics Engineering, Shizuoka University, Hamamatsu, Shizuoka 432-8561, Japan.
⁴ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
⁵ Division of Morphological Neuroscience, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan; Center for Highly Advanced Integration of Nano and Life Sciences, Gifu University, Gifu 501-1194, Japan.
⁶ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan; Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan.
⁷ Systems Neuroscience Section, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
⁸ Semel Institute for Neuroscience and Human Behavior, Intellectual Development and Disabilities Research Center, The David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
⁹ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan; Semel Institute for Neuroscience and Human Behavior, Intellectual Development and Disabilities Research Center, The David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
¹⁰ Department of Neuropsychiatry, School of Medicine, Keio University, Shinjuku, Tokyo 160-8582, Japan.
¹¹ Molecular Research Center for Children's Mental Development, United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui, Suita, Osaka 565-0871, Japan.
¹² Molecular Research Center for Children's Mental Development, United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui, Suita, Osaka 565-0871, Japan; Department of Psychiatry, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
¹³ Departments of Neuropsychiatry, Fukushima Medical University School of Medicine, Fukushima City, Fukushima 960-1295, Japan; Department of Psychiatry, Aizu Medical Center, School of Medicine, Fukushima Medical University, Aizuwakamatsu City, Fukushima 969-3492, Japan.
¹⁴ Departments of Neuropsychiatry, Fukushima Medical University School of Medicine, Fukushima City, Fukushima 960-1295, Japan.
¹⁵ The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan; Division of Bioscience, Institute for Datability Science, Osaka University, Suita, Osaka 565-0871, Japan.
¹⁶ Department of Applied Physics, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
¹⁷ Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan.
¹⁸ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan; Faculty of Pharmaceutical Sciences, Hyogo University of Health Science, Chuo-ku, Kobe, Hyogo 650-8530, Japan.
¹⁹ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan; Molecular Research Center for Children's Mental Development, United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui, Suita, Osaka 565-0871, Japan; Division of Bioscience, Institute for Datability Science, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: hasimoto@phs.osaka-u.ac.jp.

PMID: 28641108
DOI: 10.1016/j.neuron.2017.05.017

Free article

High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates

Kaoru Seiriki et al. Neuron. 2017.

Free article

. 2017 Jun 21;94(6):1085-1100.e6.

doi: 10.1016/j.neuron.2017.05.017.

Authors

Affiliations

¹ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan; Interdisciplinary Program for Biomedical Sciences, Institute for Academic Initiatives, Osaka University, Suita, Osaka 565-0871, Japan.
² Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: kasai@phs.osaka-u.ac.jp.
³ Department of Electrical & Electronics Engineering, Shizuoka University, Hamamatsu, Shizuoka 432-8561, Japan.
⁴ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
⁵ Division of Morphological Neuroscience, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan; Center for Highly Advanced Integration of Nano and Life Sciences, Gifu University, Gifu 501-1194, Japan.
⁶ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan; Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan.
⁷ Systems Neuroscience Section, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan.
⁸ Semel Institute for Neuroscience and Human Behavior, Intellectual Development and Disabilities Research Center, The David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
⁹ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan; Semel Institute for Neuroscience and Human Behavior, Intellectual Development and Disabilities Research Center, The David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
¹⁰ Department of Neuropsychiatry, School of Medicine, Keio University, Shinjuku, Tokyo 160-8582, Japan.
¹¹ Molecular Research Center for Children's Mental Development, United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui, Suita, Osaka 565-0871, Japan.
¹² Molecular Research Center for Children's Mental Development, United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui, Suita, Osaka 565-0871, Japan; Department of Psychiatry, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
¹³ Departments of Neuropsychiatry, Fukushima Medical University School of Medicine, Fukushima City, Fukushima 960-1295, Japan; Department of Psychiatry, Aizu Medical Center, School of Medicine, Fukushima Medical University, Aizuwakamatsu City, Fukushima 969-3492, Japan.
¹⁴ Departments of Neuropsychiatry, Fukushima Medical University School of Medicine, Fukushima City, Fukushima 960-1295, Japan.
¹⁵ The Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567-0047, Japan; Division of Bioscience, Institute for Datability Science, Osaka University, Suita, Osaka 565-0871, Japan.
¹⁶ Department of Applied Physics, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
¹⁷ Department of Pharmacology, Graduate School of Dentistry, Osaka University, Suita, Osaka 565-0871, Japan.
¹⁸ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan; Faculty of Pharmaceutical Sciences, Hyogo University of Health Science, Chuo-ku, Kobe, Hyogo 650-8530, Japan.
¹⁹ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan; Molecular Research Center for Children's Mental Development, United Graduate School of Child Development, Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui, Suita, Osaka 565-0871, Japan; Division of Bioscience, Institute for Datability Science, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: hasimoto@phs.osaka-u.ac.jp.

PMID: 28641108
DOI: 10.1016/j.neuron.2017.05.017

Abstract

Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases.

Keywords: cell distribution; human post-mortem brain; non-human primate brain; subcellular resolution; whole-brain imaging.

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High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates

Affiliations

High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates

Authors

Affiliations

Abstract

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LinkOut - more resources

Full Text Sources

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