Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Aug;32(4):335-337.
doi: 10.1007/s12250-017-4008-5.

A convenient immunochromatographic test strip for rapid detection of Scylla serrata reovirus

Dongyang Fan  1 , Jingwen Liu  2 , Miaomiao Xu  2 , Yangyang Yuan  1 , Jifang Yang  1 , Jigang Chen  3
Affiliations

A convenient immunochromatographic test strip for rapid detection of Scylla serrata reovirus

Dongyang Fan et al. Virol Sin. 2017 Aug.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Comparison of detection sensitivity between immunochromatographic strips (A) and one-step RT-PCR (B). (A) Homogenates from SsRV-positive S. serrata following serial dilution with MAS solution and applied to strips at a volume of 100 µL per strip. N, SsRV-negative homogenate; lanes 1–8, serially diluted SsRV-positive homogenate at 1:5, 1:10, 1:50, 1:100, 1:200, 1:400, 1:800, and 1:1000, respectively. (B) RT-PCR of homogenates from SsRV-positive S. serrata following 10-fold serial dilution with MAS solution. Lane M, DNA markers; Lane N, SsRV-negative homogenate; lanes 1–7, serially diluted SsRV-positive homogenate from 1:10 to 1:10,000,000, respectively. Arrowheads indicate the lowest amount that could be reliably detected.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Chen J, Xiong J, Cui B, et al. J Virol Method. 2011;178:153–160. doi: 10.1016/j.jviromet.2011.09.006. - DOI - PubMed
    1. Chen JG, Xiong J, Yang J, et al. Arch Virol. 2011;156:523–528. doi: 10.1007/s00705-010-0852-4. - DOI - PubMed
    1. Chen JG, Yang JF, Wang H, et al. J Mar Sci. 2008;26:93–96.
    1. Guo ZX, Weng SP, Li G, et al. J Virol Method. 2008;151:237–241. doi: 10.1016/j.jviromet.2008.05.002. - DOI - PubMed
    1. Leu JH, Tsai JM, Wang HC, et al. J Virol. 2005;79:140–149. doi: 10.1128/JVI.79.1.140-149.2005. - DOI - PMC - PubMed

LinkOut - more resources