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. 2017 Aug;32(4):335-337.
doi: 10.1007/s12250-017-4008-5.

A convenient immunochromatographic test strip for rapid detection of Scylla serrata reovirus

Dongyang Fan  1 , Jingwen Liu  2 , Miaomiao Xu  2 , Yangyang Yuan  1 , Jifang Yang  1 , Jigang Chen  3
Affiliations

A convenient immunochromatographic test strip for rapid detection of Scylla serrata reovirus

Dongyang Fan et al. Virol Sin. 2017 Aug.
No abstract available

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Figures

Figure 1
Figure 1
Comparison of detection sensitivity between immunochromatographic strips (A) and one-step RT-PCR (B). (A) Homogenates from SsRV-positive S. serrata following serial dilution with MAS solution and applied to strips at a volume of 100 µL per strip. N, SsRV-negative homogenate; lanes 1–8, serially diluted SsRV-positive homogenate at 1:5, 1:10, 1:50, 1:100, 1:200, 1:400, 1:800, and 1:1000, respectively. (B) RT-PCR of homogenates from SsRV-positive S. serrata following 10-fold serial dilution with MAS solution. Lane M, DNA markers; Lane N, SsRV-negative homogenate; lanes 1–7, serially diluted SsRV-positive homogenate from 1:10 to 1:10,000,000, respectively. Arrowheads indicate the lowest amount that could be reliably detected.
See this image and copyright information in PMC

References

    1. Chen J, Xiong J, Cui B, et al. J Virol Method. 2011;178:153–160. doi: 10.1016/j.jviromet.2011.09.006. - DOI - PubMed
    1. Chen JG, Xiong J, Yang J, et al. Arch Virol. 2011;156:523–528. doi: 10.1007/s00705-010-0852-4. - DOI - PubMed
    1. Chen JG, Yang JF, Wang H, et al. J Mar Sci. 2008;26:93–96.
    1. Guo ZX, Weng SP, Li G, et al. J Virol Method. 2008;151:237–241. doi: 10.1016/j.jviromet.2008.05.002. - DOI - PubMed
    1. Leu JH, Tsai JM, Wang HC, et al. J Virol. 2005;79:140–149. doi: 10.1128/JVI.79.1.140-149.2005. - DOI - PMC - PubMed

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