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. 2017 Jun 23;14(7):676.
doi: 10.3390/ijerph14070676.

Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples

Elena Ojer-Usoz  1 , David González  2 , Ana Isabel Vitas  3
Affiliations

Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples

Elena Ojer-Usoz et al. Int J Environ Res Public Health. .

Abstract

This study presents a comprehensive approach of a clonal diversity analysis of 448 Extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from environmental, human and food samples in Spain. The phenotypic confirmation of ESBL production was performed by disc diffusion and microdilution methods, while Polymerase Chain Reaction (PCR) and sequencing were used for the molecular characterization of β-lactamase genes (blaCTX-M, blaSHV, blaTEM, blaOXA). Clonal relationship of isolates was determined by multi-locus sequence typing (MLST). Multidrug resistant strains were present in all the studied niches, with percentages above 50.0%. The most prevalent β-lactamase genes were blaCTXM-14 (26%) and blaCTXM-1 (21.4%), followed by blaSHV-12, blaCTX-M-15 and blaTEM-42. MLST isolates were grouped into 26 clonal complexes (CC) and 177 different sequence types (ST) were detected. Despite the high clonal diversity observed, CC10 was the prevalent and the only CC detected in all niches, while other complexes as CC131 were mainly associated to human isolates. The observed prevalence and diversity of these resistant bacteria across the different environments encourages a One Health approach to prevent and control ESBL dissemination between environment and consumers.

Keywords: ESBL-producing E. coli; MLST; clonal diversity; β-lactamase genes.

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Conflict of interest statement

The authors declare no conflict of interest. The study was supported by the Research Plan of the University of Navarra (Plan de Investigación de la Universidad de Navarra (PIUNA) Project 024/2012) and a grant of the “Asociación de Amigos de la Universidad de Navarra”. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

Figures

Figure 1
Figure 1
Minimal spanning tree constructed based on the MLST profiles of the 448 ESBL-producing E. coli isolates and coloured according to ESBL genes (Bionumerics v7.5, Sint-Martens-Latem, Belgium).
Figure 2
Figure 2
Minimal spanning tree constructed based on the MLST profiles of the 448 ESBL-producing E. coli isolates and coloured according samples’ origin (Bionumerics v7.5, Sint-Martens-Latem, Belgium).
See this image and copyright information in PMC

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