Lipophilicity Influences Drug Binding to α1-Acid Glycoprotein F1/S Variants But Not to the A Variant

Abstract

Objective: Human α1-acid glycoprotein has genetic variants, the F1, S, and A variants, which can be separated isoelectrophoretically. These variants show differences in their affinity of binding to several drugs. In this study, we investigated the factors determining drug binding to these α1-acid glycoprotein genetic variants using disopyramide, warfarin, and tamsulosin as marker compounds.

Methods: Binding of the marker drugs to human α1-acid glycoprotein was determined by ultra-filtration in the presence or absence of various other drugs. For screening of the α1-acid glycoprotein variants to which the marker drugs became bound, the effects of various other drugs on their binding were studied. The binding data were analyzed using a competitive inhibition model and the relationship between the estimated dissociation constants and physicochemical properties, such as log P, was also analyzed.

Results: The binding of tamsulosin was significantly decreased by aprindine, carvedilol, erythromycin, thioridazine, and warfarin, but not by disopyramide. The dissociation constants of drugs bound to F1/S variants were significantly correlated with their lipophilicity, but those for the A variant were not.

Conclusions: We were able to develop a simple screening method for determining individual α1-acid glycoprotein variants to which drugs would bind. The binding of drugs to F1/S variants may be determined mainly by drug lipophilicity.

Fig. 1

Effects of various drugs on the binding of S-disopyramide, S-warfarin, and tamsulosin to human α1-acid glycoprotein (only typical patterns are presented). a Effect of aprindine on the binding of S-dispyramide. b Effect of aprindine on the binding of S-warfarin. c Effect of simvastatin on the binding of tamsulosin [rhombus: control, square: with test drug (9.62 µM), triangle: with test drug (19.2 µM), circle: with test drug (48.1 µM)]

Fig. 2

Relationship between partition coefficients and dissociation constants