Clinical value of miR-425-5p detection and its association with cell proliferation and apoptosis of gastric cancer

Abstract

Researches have shown that miR-425-5p expression altered in lung, esophageal, and glial cancer. Similarly, in our previous study, we found miR-425-5p expression was up-regulated in GC cells and could promote gastric cancer (GC) cell invasion and metastasis. However, the functional mechanism and the prognostic significance of miR-425-5p in GC remain unclear. Therefore, the present study examined miR-425-5p expression in GC tissues and also evaluated miR-425-5p of the therapeutic and prognostic value. Moreover, with interference of miR-425-5p expression in cell lines, we investigated the molecular mechanism of miR-425-5p and the expression level was higher in GC tissues comparing with that in gastric tumor-adjacent mucosa. These results suggested that miR-425-5p over-expression may be associated with depth of invasion and TNM stages and can be a prognostic marker of poor outcome. After inhibition of miR-425-5p expression in MKN45 cells, the cell activity was weakened. The number of G0/G1 cells increased while G2/M and S cells decreased, and the cell apoptotic rates elevated (P<0.05). Some proliferation and apoptosis related genes were altered (P<0.05). Consequently, miR-425-5p can be considered as a marker of poor prognosis and it is probably involved in GC cell proliferation and apoptosis by regulating some of the genes which participate these processes.

Keywords: Apoptosis; Biological features; Gastric carcinoma; Prognosis; Proliferation; miR-425-5p.