Postglacial dispersal patterns and mitochondrial genetic structure of the Pyrenean desman (Galemys pyrenaicus) in the northwestern region of the Iberian Peninsula

Abstract

The genetic structure of small semiaquatic animals may be influenced by dispersal across both rivers and land. The relative importance of these two modes of dispersal may vary across different species and with ecological conditions and evolutionary periods. The Pyrenean desman (Galemys pyrenaicus) is an endemic mammal of the Iberian Peninsula with a strong phylogeographic structure and semiaquatic habits, thus making it an ideal model to study the effects of river and overland dispersal on its genetic structure. Thanks to different types of noninvasive samples, we obtained an extensive sampling of the Pyrenean desman from the northwestern region of the Iberian Peninsula and sequenced two mitochondrial DNA fragments. We then analyzed, using an isolation-by-distance approach, the correlation between phylogenetic distances and geographical distances measured along both river networks and land to infer the relative importance of river and overland dispersal. We found that the correlations in the whole area and in a large basin were consistent with an effect of overland dispersal, which may be due to the postglacial colonization of new territories using terrestrial corridors and, possibly, a more extensive fluvial network that may have been present during the Holocene. However, in a small basin, likely to be less influenced by the impact of ancient postglacial dispersal, the correlations suggested significant overall effects of both overland and river dispersal, as expected for a semiaquatic mammal. Therefore, different scales and geographical regions reflect different aspects of the evolutionary history and ecology of this semiaquatic species using this isolation-by-distance method. The results we obtained may have crucial implications for the conservation of the Pyrenean desman because they reinforce the importance of interbasin dispersal for this species in the studied area and the need to protect the whole riverine ecosystem, including rivers, upland streams and terrestrial corridors between basins.

Keywords: Galemys pyrenaicus; Iberian Peninsula; dispersal; genetic diversity; isolation‐by‐distance; mitochondrial DNA.

Figure 1

Scheme of the computation of Euclidean (a), least‐cost path (b), and river (c) distances between two sampling points

Figure 2

Haplotype genealogy of the mitochondrial sequences based on a maximum‐likelihood tree in which circles represent haplotypes, size is proportional to the number of individuals, and black dots represent intermediate, unsampled haplotypes (a), and map of the samples colored according to the mitochondrial clade (b)

Figure 3

Plot of interpolated nucleotide diversity values, with the color scale indicating nucleotide diversity. Different haplotypes are shown with different colors. A different random color is used for each haplotype, and therefore, a greater variety of colors in an area also indicates greater genetic diversity