Assembly of the mannose-6-phosphate receptor into reconstituted clathrin coats
- PMID: 2865131
- PMCID: PMC554529
- DOI: 10.1002/j.1460-2075.1985.tb03956.x
Assembly of the mannose-6-phosphate receptor into reconstituted clathrin coats
Abstract
In ionic conditions in which clathrin coats are stable, the mannose-6-phosphate receptor associates with the 100-kd/50-kd coat complexes purified from bullock brain coated vesicles. These aggregates exist as striking spherical structures of 300-1000 A diameter. When clathrin is included in the assembly mixture, cages are formed which apparently encapsulate these aggregates, giving, in the absence of lipid, structures resembling full coated vesicles.
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