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. 2017 Mar:66:68-75.
doi: 10.1016/j.idairyj.2016.10.016. Epub 2016 Nov 21.

Role of pH in the recovery of bovine milk oligosaccharides from colostrum whey permeate by nanofiltration

Joshua L Cohen  1 , Daniela Barile  1   2 , Yan Liu  1 , Juliana M L N de Moura Bell  1
Affiliations

Role of pH in the recovery of bovine milk oligosaccharides from colostrum whey permeate by nanofiltration

Joshua L Cohen et al. Int Dairy J. 2017 Mar.

Abstract

Milk oligosaccharides are associated with improved health outcomes in infants. Nanofiltration (NF) is used for isolation of bovine milk oligosaccharides (BMO). The study aim was to improve the recovery of BMO from lactose-hydrolyzed colostrum whey permeate. The retention factors of carbohydrates at various pH and transmembrane pressures were determined for a nanofiltration membrane, which was used at pilot scale to purify BMO. Carbohydrates were quantified by liquid chromatography and characterized using nano-LC-Chip-QToF mass spectrometry. BMO purity was improved from an initial 4% in colostrum whey permeate to 98%, with 99.8% permeation of monosaccharides and 96% recovery of oligosaccharides, represented by 23 unique BMO compounds identified in the final retentate. The pH during NF was a determining factor in the selectivity of carbohydrate separation. This NF method can be applied to conventional cheese-whey permeate and other milk types for extraction of bioactive oligosaccharides providing new options for the dairy industry.

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Figures

Fig. 1
Fig. 1
Effects of transmembrane pressure (TMP) and pH (■, pH 4.5; □, pH 7; ×, pH 8.5) on the retention of the hydrolyzed colostrum whey permeate monosaccharides (A) glucose and (B) galactose (500–700 Da membrane). Error bars represent one standard deviation.
Fig. 2
Fig. 2
Effects of transmembrane pressure (TMP) and pH (■, pH 4.5; □, pH 7; ×, pH 8.5) on the retention of the hydrolyzed colostrum whey permeate oligosaccharides (A) 3’-sialyllactose (3’-SL), (B) 6’-sialyllactose (6’-SL) and (C) 6’-sialyllactosamine (6’-SLN).
Fig. 3
Fig. 3
Effects of transmembrane pressure (TMP) and pH (■, pH 4.5; □, pH 7; ×, pH 8.5) on permeate flux of nanofiltered colostrum whey permeate (500–700 Da membrane). Error bars represent one standard deviation.
Fig. 4
Fig. 4
Effects of concentration factor on colostrum whey permeate flux (▽, monosaccharides (●) and oligosaccharide (■) yields in the nanofiltration of hydrolyzed colostrum whey permeate (500–700 Da membrane, at 20 bar, 50 °C). Error bars represent one standard deviation.
Fig. 5
Fig. 5
Nano-liquid chromatography Chip quadrupole time of flight mass spectrometry (Nano-LC-chip Q-ToF): ■, hydrolyzed colostrum whey permeate; □, final retentate. Integrated peak areas of extracted compound chromatograms identified by Mass Hunter Profinder’s “Batch Targeted Feature Extraction” algorithm using an in-house bovine milk oligosaccharide bioformatic library. Component nomenclature is based upon the number of monosaccharide residues in the order of Hex- HexNAc-Fuc-NeuAc-NeuGc; where Hex, hexose; HexNAc, N-acetylhexosamine; Fuc, fucose; NeuAc, N-acetylneuraminic acid; NeuGc, N-glycolylneuraminic acid.
See this image and copyright information in PMC

References

    1. Aldredge DL, Geronimo MR, Hua S, Nwosu CC, Lebrilla CB, Barile D. Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures. Glycobiology. 2013;23:664–676. - PMC - PubMed
    1. Altmann K, Wutkowski A, Kämpfer S, Klempt M, Lorenzen PC, Clawin-Rädecker I. Comparison of the efficiency of different NF membranes for the enrichment of milk oligosaccharides from bovine milk. European Food Research and Technology. 2015;24:803–815.
    1. Barile D, Marotta M, Chu C, Mehra R, Grimm R, Lebrilla CB, et al. Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry. Journal of Dairy Science. 2010;93:3940–3949. - PMC - PubMed
    1. Cheryan M. Ultrafiltration and microfiltration handbook. Boca Raton, FL, USA: CRC Press; 1998.
    1. Corradini C, Cavazza A, Bignardi C. High-performance anion-exchange chromatography coupled with pulsed electrochemical detection as a powerful tool to evaluate carbohydrates of food interest: Principles and applications. International Journal of Carbohydrate Chemistry. 2012;201 Article no. 487564.