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. 2018:1577:307-315.
doi: 10.1007/7651_2017_32.

Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure

Steven Skolasinski  1 , Angela Panoskaltsis-Mortari  2
Affiliations

Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure

Steven Skolasinski et al. Methods Mol Biol. 2018.

Abstract

Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

Keywords: Decellularization; Lung; Negative pressure bioreactor; Organ scaffold.

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Figures

Fig. 1
Fig. 1
Photo of Ventilator Reservoir. Lines are drawn over tubing in this image to allow the path to be followed inside the flask.
Fig. 2
Fig. 2
Lung with barb connectors in the Pulmonary Artery (left) and Trachea (right)
Fig. 3
Fig. 3
Bioreactor assembly
Fig. 4
Fig. 4
Photo of decellularization system with close-up of the top of the bioreactor showing the connectors
See this image and copyright information in PMC

References

    1. Price AP, England KA, Matson AM, et al. Development of a decellularized lung bioreactor system for bioengineering the lung: the matrix reloaded. Tissue Eng Part A. 2010;16:2581–91. doi: 10.1089/ten.TEA.2009.0659. - DOI - PMC - PubMed
    1. Crabbé A, Liu Y, Sarker SF, et al. Recellularization of decellularized lung scaffolds is enhanced by dynamic suspension culture. PLoS One. 2015;10:1–17. doi: 10.1371/journal.pone.0126846. - DOI - PMC - PubMed
    1. Nichols JE, La Francesca S, Vega SP, et al. Giving new life to old lungs: methods to produce and assess whole human paediatric bioengineered lungs. J Tissue Eng Regen Med. 2016 doi: 10.1002/term.2113. n/a-n/a. - DOI - PubMed
    1. Franks TJ, Colby TV, Travis WD, et al. Resident Cellular Components of the Human Lung: Current Knowledge and Goals for Research on Cell Phenotyping and Function. Proc Am Thorac Soc. 2008;5:763–766. doi: 10.1513/pats.200803-025HR. - DOI - PubMed
    1. Stone KC, Mercer RR, Freeman BA, et al. Distribution of Lung Cell Numbers and Volumes between Alveolar and Nonalveolar Tissue. Am Rev Respir Dis. 1992;146:454–456. doi: 10.1164/ajrccm/146.2.454. - DOI - PubMed