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. 2018:1577:307-315.
doi: 10.1007/7651_2017_32.

Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure

Steven Skolasinski  1 , Angela Panoskaltsis-Mortari  2
Affiliations

Decellularization of Intact Lung Tissue Through Vasculature and Airways Using Negative and Positive Pressure

Steven Skolasinski et al. Methods Mol Biol. 2018.

Abstract

Decellularization allows the production of extracellular matrix (ECM) scaffolds. Here we describe the use of combined positive pressure and negative pressure to drive decellularization reagents into the vasculature and airways, respectively, of structurally intact lungs in order to remove cells and cellular material leaving an intact ECM scaffold.

Keywords: Decellularization; Lung; Negative pressure bioreactor; Organ scaffold.

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Figures

Fig. 1
Fig. 1
Photo of Ventilator Reservoir. Lines are drawn over tubing in this image to allow the path to be followed inside the flask.
Fig. 2
Fig. 2
Lung with barb connectors in the Pulmonary Artery (left) and Trachea (right)
Fig. 3
Fig. 3
Bioreactor assembly
Fig. 4
Fig. 4
Photo of decellularization system with close-up of the top of the bioreactor showing the connectors
See this image and copyright information in PMC

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