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. 2017 Jul;7(3):164.
doi: 10.1007/s13205-017-0834-2. Epub 2017 Jun 29.

Bioactive profile, free-radical scavenging potential, DNA damage protection activity, and mycochemicals in Aspergillus awamori (MTCC 548) extracts: a novel report on filamentous fungi

Raj Kumar Salar  1 , Sukhvinder Singh Purewal  2 , Kawaljit Singh Sandhu  3
Affiliations

Bioactive profile, free-radical scavenging potential, DNA damage protection activity, and mycochemicals in Aspergillus awamori (MTCC 548) extracts: a novel report on filamentous fungi

Raj Kumar Salar et al. 3 Biotech. 2017 Jul.

Abstract

Biomass of Aspergillus awamori was investigated for mycochemicals, total phenolic compounds (TPC), condensed tannin content (CTC), free-radical scavenging potential (FRSP), and DNA damage protection activity. FRSP was determined using DPPH, ABTS, FRAP (Ferric reducing antioxidant power), metal chelating activity, and cupric reducing antioxidant capacity) assays. Water (Aq), aqueous ethanol 50% (AqE), and methanol were used as extraction phase at 44.5 °C for 23.8 min. AqE shows the presence of maximum mycochemicals (coumarins, glucose, saponins, flavonoids, and tannin). Further quantitative analysis shows maximum TPC (23.17 mg GAE/g dwb) in AqE and CTC (.89 mg CE/g dwb) in ME. Qualitative and quantitative analysis for identification of specific bioactive compound in AqE was carried out using HPLC. HPLC analysis confirmed the presence of bioactive compounds: p'-Coumaric acid (5.96 mg/g dwb), cinnamic acid (4.31 mg/g dwb), gallic acid (2.27 mg/g dwb), and ascorbic acid (.98 mg/g dwb). All the extracts show significant DNA damage protection activity; however, AqE showed the maximum activity. Pearson correlations were also calculated to find the relationships between bioactive compounds and antioxidant potential.

Keywords: DNA protection; GRAS fungi; HPLC; Mycochemicals; Phenolic compounds.

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Conflict of interest statement

Authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Total phenolic compounds (mg GAE/g dwb) in different extracts
Fig. 2
Fig. 2
Condensed tannin content (mg GAE/g dwb) in different extracts
Fig. 3
Fig. 3
HPLC chromatogram of Aspergillus awamori extract from AqE (50%) (above) and standard phenolic compounds (below)
Fig. 4
Fig. 4
DNA damage protecting activity in different extracts of Aspergillus awamori (MTCC 548) against hydroxyl radicals induced DNA damage of pBR322. Lane 1 Native pBR322 plasmid DNA, Lane 2 DNA + Fenton’s reagent + Quercetin (mg/ml positive control), Lane 3 DNA + Fenton’s reagent, Lane 4 DNA + Fenton’s reagent + ethanol 50% extract, and Lane 5 DNA + Fenton’s reagent + Water extract, Lane 6 DNA + Fenton’s reagent + methanol extract
Fig. 5
Fig. 5
Principal control analysis: Score plot of first principal component and second principal component
Fig. 6
Fig. 6
Principal component analysis: loading plot of PC1 and PC2 describing the relationship among TPC, CTC, and antioxidant potential of Aspergillus awamori
See this image and copyright information in PMC

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