[Normal and modified nucleobases excreted in urine of patients with chronic nyeloproliferative disorders (author's transl)]

Abstract

The qualitative and quantitative analysis by high performance liquid chromatography of the normal and modiefied nucleobases excreted in urine represents a new and versatile tool, especially in oncology. The excretion of 2 normal (adenine, guanine) and 4 modified nucleobases (methylated guanine derivatives) in urine was measured by cation exchange LC. All chronic myeloproliferative syndromes showed highly elevated total excretion values of all determined nucleobases, the "pattern" being characteristic with N2, N2-dimethylguanine most prominent (up to 29.8 S.D. above the pertinent normal value). A follow-up study of a case of CML with two episodes of extreme leukocytosis showed a correlation of the nucleobases excretion with the number of leukocytes. Thus, a method has been established which permits the assessment of myeloproliferation and probably therapy effects.