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. 1985 Nov;17(2):163-70.
doi: 10.1016/0166-6851(85)90015-5.

Cell free synthesis of Toxoplasma gondii antigens

J B Prince, M A Koven-Quinn, J S Remington, S D Sharma

Cell free synthesis of Toxoplasma gondii antigens

J B Prince et al. Mol Biochem Parasitol. 1985 Nov.

Abstract

Functionally active poly(A)-containing mRNA was isolated from tachyzoites of the RH strain of Toxoplasma gondii. The T. gondii mRNA was capable of directing the synthesis of proteins in a wheat germ in vitro translation system, but not in a cell free system derived from rabbit reticulocyte lysate. Efficient translation in the wheat germ system required spermine and exogenous tRNA. Amino acid incorporation was maximal at 110 mM K+ and 1.8 mM Mg2+. Tachyzoite antigens synthesized in vitro were immunoprecipitated with T. gondii antibodies from rabbits, mice and humans. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of immunoprecipitated polypeptides yielded patterns that differed according to antibody source, but all T. gondii antibody preparations reacted with a translation product with an apparent molecular weight of 24 000.

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