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. 1985 Nov 27;133(1):280-5.
doi: 10.1016/0006-291x(85)91872-8.

Fusicoccin stimulates the H+-ATPase of plasmalemma in isolated membrane vesicles from radish

F Rasi-Caldogno, M C Pugliarello

Fusicoccin stimulates the H+-ATPase of plasmalemma in isolated membrane vesicles from radish

F Rasi-Caldogno et al. Biochem Biophys Res Commun. .

Abstract

The effect of fusicoccin on the plasmalemma H+-ATPase has been investigated in a membrane fraction from 24 h old radish seedlings, in which Mg:ATP-dependent H+-transport is mediated only by the plasmalemma H+-ATPase. Fusicoccin stimulated the plasmalemma H+-ATPase - i.e. Mg:ATP-dependent intravesicular acidification, hyperpolaryzation of delta psi and ATPase activity -, when these activities were measured at the physiologically relevant pHs of 7.3 to 7.6. No effect of FC on the plasmalemma H+-ATPase was evident at pH 6.6.

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