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. 2017 Aug 24;61(9):e00877-17.
doi: 10.1128/AAC.00877-17. Print 2017 Sep.

First Report of Klebsiella oxytoca Strain Simultaneously Producing NDM-1, IMP-4, and KPC-2 Carbapenemases

Juan Wang  1   2 , Min Yuan  3 , Hai Chen  4 , Xia Chen  3 , Yuanchun Jia  3 , Xiong Zhu  4 , Li Bai  5 , Xue Bai  1 , Séamus Fanning  5   2   6 , Jinxing Lu  3 , Juan Li  7
Affiliations

First Report of Klebsiella oxytoca Strain Simultaneously Producing NDM-1, IMP-4, and KPC-2 Carbapenemases

Juan Wang et al. Antimicrob Agents Chemother. .

Abstract

The nucleotide sequences of five plasmids from one Klebsiella oxytoca isolate were determined using the PacBio RS II system. Plasmid analysis revealed that blaNDM-1 was carried on an IncX3 plasmid. The blaIMP-4 and blaKPC-2 genes were located on IncN and IncP-6 plasmids, respectively. Comparative sequence analysis highlighted the successful spread of carbapenemase-harboring plasmids among different enterobacterial species. We report for the first time, to our knowledge, coproducing NDM-1, KPC-2, and IMP-4 carbapenemases on a K. oxytoca isolate.

Keywords: KPC-2; MBLs; MDR Klebsiella oxytoca; carbapenemases; plasmids.

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Figures

FIG 1
FIG 1
(a) Schematic illustration comparing structural features of plasmid pKOX3-P3-NDM with sequences of plasmids p112298-NDM (GenBank accession no. KP987216) and pNDM-HN380 (GenBank accession no. JX104760). (b) Schematic illustration comparing structural features of plasmid pKOX3-P4-IMP with sequences of plasmids p378-IMP (GenBank accession no. KX711879) and pIMP-HZ1 (GenBank accession no. KU886034). (c) Schematic illustration comparing structural features of plasmid pKOX3-P5-KPC with sequences of plasmids p10265-KPC (GenBank accession no. KU578314) and pKCP2 (GenBank accession no. KR014106). Gray shading indicates homologies between the corresponding genetic loci on each plasmid. Arrows indicate open reading frames, with arrowheads indicating the direction of transcription: red, antibiotic resistance-encoding genes; yellow, individual conjugation-related genes (associated with tra and trh); blue, transposon- and integron-associated genes; green, replication-associated genes; gray, heavy metal resistance genes, maintenance/stability functioning genes, or hypothetical proteins. The figure is not drawn to scale.
See this image and copyright information in PMC

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