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. 2017 Aug 24;61(9):e00818-17.
doi: 10.1128/AAC.00818-17. Print 2017 Sep.

OXA-244-Producing Escherichia coli Isolates, a Challenge for Clinical Microbiology Laboratories

Yannick Hoyos-Mallecot  1   2   3 , Thierry Naas  4   2   3   5 , Rémy A Bonnin  1   2   3   5 , Rafael Patino  5 , Philippe Glaser  5 , Nicolas Fortineau  1   2   3   5 , Laurent Dortet  1   2   3   5
Affiliations

OXA-244-Producing Escherichia coli Isolates, a Challenge for Clinical Microbiology Laboratories

Yannick Hoyos-Mallecot et al. Antimicrob Agents Chemother. .

Abstract

OXA-244 is a single-point-mutant derivative of OXA-48 displaying reduced carbapenemase activity. Here, we report the microbiological features of seven OXA-244-producing Escherichia coli isolates. Only one isolate grew on ChromID Carba Smart medium (bioMérieux), but six of the seven isolates grew on ChromID extended-spectrum-β-lactamase (ESBL) medium (bioMérieux), as they coproduced an ESBL and/or a plasmid-encoded cephalosporinase. The production of a carbapenemase was detected in 57.1%, 71.4%, 71.4%, and 100% of the E. coli isolates using the Carba NP test, the Rapidec Carba NP test (bioMérieux), a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) hydrolysis assay (Bruker), and the OXA-48 K-SeT assay (Coris BioConcept), respectively. Our results indicate that OXA-244-producing E. coli isolates are difficult to detect, which may lead to their silent spread.

Keywords: OXA-244; OXA-48-like; carbapenemase activity; detection; screening; tests.

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Figures

FIG 1
FIG 1
Rep-PCR analysis using the DiversiLab technique, showing a dendrogram and computer-generated image of rep-PCR banding patterns of OXA-244-Ec isolates and an E. coli isolate of an unrelated strain. As recommended by the manufacturer, a cutoff value of 95% similarity defined a cluster.
See this image and copyright information in PMC

References

    1. Doi Y, Paterson DL. 2015. Carbapenemase-producing Enterobacteriaceae. Semin Respir Crit Care Med 36:74–84. doi:10.1055/s-0035-1544208. - DOI - PMC - PubMed
    1. Grundmann H, Livermore DM, Giske CG, Canton R, Rossolini GM, Campos J, Vatopoulos A, Gniadkowski M, Toth A, Pfeifer Y, Jarlier V, Carmeli Y. 2010. Carbapenem-non-susceptible Enterobacteriaceae in Europe: conclusions from a meeting of national experts. Euro Surveill 15(46):pii=19711 http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19711. - PubMed
    1. Viau R, Frank KM, Jacobs MR, Wilson B, Kaye K, Donskey CJ, Perez F, Endimiani A, Bonomo RA. 2016. Intestinal carriage of carbapenemase-producing organisms: current status of surveillance. Methods Clin Microbiol Rev 29:1–27. doi:10.1128/CMR.00108-14. - DOI - PMC - PubMed
    1. Oteo J, Hernández JM, Espasa M, Fleites A, Sáez D, Bautista V, Pérez-Vázquez M, Fernández-García MD, Delgado-Iribarren A, Sánchez-Romero I, García-Picazo L, Miguel MD, Solís S, Aznar E, Trujillo G, Mediavilla C, Fontanals D, Rojo S, Vindel A, Campos J. 2013. Emergence of OXA-48-producing Klebsiella pneumoniae and the novel carbapenemases OXA-244 and OXA-245 in Spain. J Antimicrob Chemother 68:317–321. doi:10.1093/jac/dks383. - DOI - PubMed
    1. Valenza G, Nickel S, Pfeifer Y, Eller C, Krupa E, Lehner-Reindl V, Höller C. 2014. Extended-spectrum-β-lactamase-producing Escherichia coli as intestinal colonizers in the German community. Antimicrob Agents Chemother 58:1228–1230. doi:10.1128/AAC.01993-13. - DOI - PMC - PubMed

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