doi: 10.1016/0003-2697(85)90465-8.
Microdetermination of serum D-amino acids
- PMID: 2867715
- DOI: 10.1016/0003-2697(85)90465-8
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Microdetermination of serum D-amino acids
Anal Biochem.
1985 Oct.
Abstract
A method for the quantitative determination of serum D-amino acids in the range 0.5-20 nmol is described. In the method alpha-keto acids, derived from D-amino acids by D-amino acid oxidase, are measured as hydrazones. The method is unresponsive to the presence of a large excess of L-amino acids. It allows a fast assay in a small amount of specimen (0.1 ml), with good reproducibility and accuracy.
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