Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jul 5:80:9.59.1-9.59.10.
doi: 10.1002/cpns.31.

Cannabinoid-Induced Tetrad in Mice

Mathilde Metna-Laurent  1 , Miguel Mondésir  2   3 , Agnès Grel  2   3 , Monique Vallée  2   3 , Pier-Vincenzo Piazza  2   3
Affiliations

Cannabinoid-Induced Tetrad in Mice

Mathilde Metna-Laurent et al. Curr Protoc Neurosci. .

Abstract

Cannabinoid-induced tetrad is a preclinical model commonly used to evaluate if a pharmacological compound is an agonist of the central type-1 cannabinoid (CB1) receptor in rodents. The tetrad is characterized by hypolocomotion, hypothermia, catalepsy, and analgesia, four phenotypes that are induced by acute administration of CB1 agonists exemplified by the prototypic cannabinoid delta-9-tetrahydrocannabinol (THC). This unit describes a standard protocol in mice to induce tetrad phenotypes with THC as reference cannabinoid. We provide typical results obtained with this procedure showing a dose effect of THC in different mouse strains. The effect of the CB1 antagonist rimonabant is also shown. This tetrad protocol is well adapted to reveal new compounds acting on CB1 receptors in vivo. © 2017 by John Wiley & Sons, Inc.

Keywords: CB1 receptors; cannabinoid; mice; tetrad.

PubMed Disclaimer

References

Literature Cited

    1. Ahn, K. H., Mahmoud, M. M., Shim, J.-Y., & Kendall, D. A. (2013). Distinct roles of β-arrestin 1 and β-arrestin 2 in ORG27569-induced biased signaling and internalization of the cannabinoid receptor 1 (CB1). Journal of Biological Chemistry, 288, 9790-9800. doi: 10.1074/jbc.M112.438804.
    1. Bénard, G., Massa, F., Puente, N., Lourenço, J., Bellocchio, L., Soria-Gómez, E., … Marsicano, G. (2012). Mitochondrial CB1 receptors regulate neuronal energy metabolism. Nature Neuroscience, 15, 558-564. doi: 10.1038/nn.3053.
    1. Compton, D. R., Aceto, M. D., Lowe, J., & Martin, B. R. (1996). In vivo characterization of a specific cannabinoid receptor antagonist (SR141716A): Inhibition of delta 9-tetrahydrocannabinol-induced responses and apparent agonist activity. The Journal of Pharmacology and Experimental Therapeutics, 277, 586-594.
    1. Compton, D. R., Rice, K. C., De Costa, B. R., Razdan, R. K., Melvin, L. S., Johnson, M. R., & Martin, B. R. (1993). Cannabinoid structure-activity relationships: Correlation of receptor binding and in vivo activities. The Journal of Pharmacology and Experimental Therapeutics, 265, 218-226.
    1. DeLong, G. T., Wolf, C. E., Poklis, A., & Lichtman, A. H. (2010). Pharmacological evaluation of the natural constituent of Cannabis sativa, cannabichromene and its modulation by Δ(9)-tetrahydrocannabinol. Drug and Alcohol Dependence, 112, 126-133. doi: 10.1016/j.drugalcdep.2010.05.019.

LinkOut - more resources