Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Feb;165(2):625-30.
doi: 10.1128/jb.165.2.625-630.1986.

Expression of the Pseudomonas aeruginosa PAK pilin gene in Escherichia coli

B B Finlay, B L Pasloske, W Paranchych

Expression of the Pseudomonas aeruginosa PAK pilin gene in Escherichia coli

B B Finlay et al. J Bacteriol. 1986 Feb.

Abstract

Pseudomonas aeruginosa is a piliated opportunistic pathogen. We have recently reported the cloning of the structural gene for the pilus protein, pilin, from P. aeruginosa PAK (B. L. Pasloske, B. B. Finlay, and W. Paranchych, FEBS Lett. 183:408-412, 1985), and in this paper we present evidence that this chimera (pBP001) expresses P. aeruginosa PAK pilin in Escherichia coli independent of a vector promoter. The strength of the promoter for the PAK pilin gene was assayed, and the cellular location of the pilin protein within E. coli was examined. This protein was present mainly in the inner membrane fraction both with and without its six-amino-acid leader sequence, but it was not assembled into pili.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Annu Rev Genet. 1979;13:319-53 - PubMed
    1. J Biochem. 1975 Apr;77(4):705-18 - PubMed
    1. J Bacteriol. 1982 Nov;152(2):687-91 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6110-4 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources