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. 2017 Jul 5;547(7661):E7-E8.
doi: 10.1038/nature22963.

Upholding a role for EMT in pancreatic cancer metastasis

Nicole M Aiello  1 , Thomas Brabletz  2 , Yibin Kang  3 , M Angela Nieto  4 , Robert A Weinberg  5   6   7 , Ben Z Stanger  1
Affiliations

Upholding a role for EMT in pancreatic cancer metastasis

Nicole M Aiello et al. Nature. .
No abstract available

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Conflict of interest statement

Competing Financial Interests Declared none

Figures

Figure 1
Figure 1
Six pancreatic tumours from KPCY mice (4) were stained for YFP (tumour cells, red) and the mesenchymal markers Fsp1, vimentin (Vim), Zeb1, or αSMA (green). The analysis included three poorly differentiated tumors (with a high degree of EMT) and three well differentiated tumors (with a low degree of EMT) to reflect the heterogeneity seen in the KPCY model; all images were taken from the poorly differentiated tumors. Although there was wide variation in the frequency of Fsp1, Vimentin, and Zeb1 staining in the tumour cells, virtually all of the αSMA staining was confined to the non-tumour (YFP-negative) stroma. This was true in both well-differentiated and poorly differentiated tumours (arrows indicate YFP+ tumour cells expressing the indicated marker; αSMA+YFP+ cells were extremely rare). These data were quantified in the graph below, demonstrating that αSMA expression is uncommon in the pancreatic carcinoma cells including those that have undergone an EMT. A minimum of 2,500 tumour cells were counted per mouse for each marker.
See this image and copyright information in PMC

References

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