Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jul 5;547(7661):E7-E8.
doi: 10.1038/nature22963.

Upholding a role for EMT in pancreatic cancer metastasis

Nicole M Aiello  1 , Thomas Brabletz  2 , Yibin Kang  3 , M Angela Nieto  4 , Robert A Weinberg  5   6   7 , Ben Z Stanger  1
Affiliations

Upholding a role for EMT in pancreatic cancer metastasis

Nicole M Aiello et al. Nature. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

Competing Financial Interests Declared none

Figures

Figure 1
Figure 1
Six pancreatic tumours from KPCY mice (4) were stained for YFP (tumour cells, red) and the mesenchymal markers Fsp1, vimentin (Vim), Zeb1, or αSMA (green). The analysis included three poorly differentiated tumors (with a high degree of EMT) and three well differentiated tumors (with a low degree of EMT) to reflect the heterogeneity seen in the KPCY model; all images were taken from the poorly differentiated tumors. Although there was wide variation in the frequency of Fsp1, Vimentin, and Zeb1 staining in the tumour cells, virtually all of the αSMA staining was confined to the non-tumour (YFP-negative) stroma. This was true in both well-differentiated and poorly differentiated tumours (arrows indicate YFP+ tumour cells expressing the indicated marker; αSMA+YFP+ cells were extremely rare). These data were quantified in the graph below, demonstrating that αSMA expression is uncommon in the pancreatic carcinoma cells including those that have undergone an EMT. A minimum of 2,500 tumour cells were counted per mouse for each marker.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Zheng X, et al. Epithelial-to-mesenchymal transition is dispensable for metastasis but induces chemoresistance in pancreatic cancer. Nature. 2015;527:525–530. doi: 10.1038/nature16064. - DOI - PMC - PubMed
    1. Nieto MA. The ins and outs of the epithelial to mesenchymal transition in health and disease. Annu Rev Cell Dev Biol. 2011;27:347–376. doi: 10.1146/annurev-cellbio-092910-154036. - DOI - PubMed
    1. Nieto MA. Epithelial plasticity: a common theme in embryonic and cancer cells. Science. 2013;342:1234850. doi: 10.1126/science.1234850. - DOI - PubMed
    1. Rhim AD, et al. EMT and dissemination precede pancreatic tumor formation. Cell. 2012;148:349–361. doi: 10.1016/j.cell.2011.11.025. - DOI - PMC - PubMed