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. 2017 Jul 6;10(1):249.
doi: 10.1186/s13104-017-2565-1.

Identification and characterization of Burkholderia multivorans CCA53

Hironaga Akita  1 , Zen-Ichiro Kimura  2 , Mohd Zulkhairi Mohd Yusoff  3   4 , Nobutaka Nakashima  5   6 , Tamotsu Hoshino  3   5
Affiliations

Identification and characterization of Burkholderia multivorans CCA53

Hironaga Akita et al. BMC Res Notes. .

Abstract

Objective: A lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53.

Results: Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences was performed to identify Burkholderia sp. CCA53. The MLST analysis revealed that Burkholderia sp. CCA53 was tightly clustered with B. multivorans ATCC BAA-247T. The quinone and cellular fatty acid profiles, carbon source utilization, growth temperature and pH were consistent with the characteristics of B. multivorans species. Burkholderia sp. CCA53 was therefore identified as B. multivorans CCA53.

Keywords: Burkholderia multivorans; Lignin-degrading bacterium; MLST analysis; Second-generation biofuel.

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Figures

Fig. 1
Fig. 1
Phylogenetic tree reconstructed from analysis of the sequences of six housekeeping genes (atpD, gltD, gyrB, lepA, recA and trpB) and showing the relationship of CCA53 with related species. Pandoraea apista DSM 16536T was used as an outgroup. The tree was reconstructed using the neighbor-joining method with Kimura’s two-parameter model [28]. Closed circles show the nodes supported by 80% bootstrap probabilities with 1000 replicates. The bar indicates the 1% nucleotide substitution rate
Fig. 2
Fig. 2
Effects of temperature and pH on growth of B. multivorans CCA53. Cells were cultured in Nutrient Broth. Error bars indicate SE (n = 3)
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