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. 2017 Dec;245(Pt B):1641-1644.
doi: 10.1016/j.biortech.2017.06.105. Epub 2017 Jun 23.

Enhancing linalool production by engineering oleaginous yeast Yarrowia lipolytica

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Enhancing linalool production by engineering oleaginous yeast Yarrowia lipolytica

Xuan Cao et al. Bioresour Technol. 2017 Dec.

Abstract

In this study, stepwise increases in linalool production were obtained by combining metabolic engineering and process optimization of an unconventional oleaginous yeast Yarrowia lipolytica. The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica. To enhance linalool productivity, key genes involved in the mevalonate pathway were overexpressed in different combinations. Moreover, the overexpression of mutant ERG20F88W-N119W gene resulted in further linalool production. A maximum linalool titre of 6.96±0.29mg/L was achieved in shake flasks, which was the highest level ever reported in yeasts.

Keywords: Linalool; Linalool synthase; MVA pathway; Yarrowia lipolytica.

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