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. 2017 Jul;14(1):1068-1072.
doi: 10.3892/ol.2017.6186. Epub 2017 May 17.

Long non-coding RNA HOXA transcript at the distal tip as a biomarker for gastric cancer

Yuchong Yang  1 , Bin Ma  1 , Ying Yan  2 , Yongxi Song  1 , Peng Gao  1 , Jingxu Sun  1 , Jiajun Wang  1 , Zhenning Wang  1
Affiliations

Long non-coding RNA HOXA transcript at the distal tip as a biomarker for gastric cancer

Yuchong Yang et al. Oncol Lett. 2017 Jul.

Abstract

A long non-coding RNA named HOXA transcript at the distal tip (HOTTIP) has been reported to be significantly increased in several cancers, including hepatocellular cancer, pancreatic cancer and lung cancer. However, the clinical value of HOTTIP expression in gastric cancer remains unknown. The present study aimed to investigate HOTTIP expression levels in gastric cancer and to elucidate its clinical significance. Reverse transcription polymerase chain reaction was used to assess the expression level of HOTTIP in gastric cancer cell lines and tissues. In a cohort of 94 patients with gastric cancer, HOTTIP expression was significantly lower in cancer tissues compared with the normal adjacent tissues. In addition, receiver operating characteristic (ROC) curve analysis was performed to evaluate the diagnostic value of HOTTIP in gastric cancer, and the area under the ROC curve was 0.767. In conclusion, the results of the present study indicated that HOTTIP may be a predictive biomarker for gastric cancer.

Keywords: HOXA transcript at the distal tip; biomarker; gastric cancer; long non-coding RNA.

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Figures

Figure 1.
Figure 1.
Expression of HOTTIP in five gastric cancer cell lines. Quantification of HOTTIP was detected by reverse transcription-quantitative polymerase chain reaction. Data are presented in gastric cancer cell lines relative to three NATs randomly selected from 94 patients as a control. Data were expressed as the mean ± standard deviation from at least three separate experiments. **P<0.01 vs. NATs. HOTTIP, HOXA transcript at the distal tip; NATs, normal adjacent tissues.
Figure 2.
Figure 2.
Expression of HOTTIP in 94 patients with gastric cancer. (A) Data were presented as log2 of fold-change of gastric cancer tissues relative to NATs. Each case was analyzed in triplicate and repeated three times. (B) ΔCq was used to compare the relative expression of HOTTIP in cancer tissues and NATs. The results indicated that the value of ΔCq was significantly higher in gastric cancer tissues compared with NATs. Data were expressed as the mean ± standard deviation from at least three separate experiments. Larger ΔCq values indicated lower expression. **P<0.01 vs. NATs. HOTTIP, HOXA transcript at the distal tip; NATs, normal adjacent tissues.
Figure 3.
Figure 3.
HOTTIP expression and survival. (A) Kaplan-Meier analysis of overall survival based on HOTTIP expression in all 94 patients with gastric cancer (P=0.860). (B) Kaplan-Meier analysis of disease-free survival based on HOTTIP expression in all 94 patients with gastric cancer (P=0.427). HOTTIP, HOXA transcript at the distal tip.
Figure 4.
Figure 4.
ROC curve based on HOXA transcript at the distal tip expression in cancer tissues and normal adjacent tissues. The AUC was 0.767 (P<0.001). ROC, Receiver operating characteristic; AUC, area under ROC curve.
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