Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017:2017:3649693.
doi: 10.1155/2017/3649693. Epub 2017 Jun 14.

Overexpression of miR-24 Is Involved in the Formation of Hypocoagulation State after Severe Trauma by Inhibiting the Synthesis of Coagulation Factor X

Lu-Jia Chen  1 , Lian Yang  2 , Xing Cheng  1 , Yin-Kai Xue  1 , Li-Bo Chen  1
Affiliations

Overexpression of miR-24 Is Involved in the Formation of Hypocoagulation State after Severe Trauma by Inhibiting the Synthesis of Coagulation Factor X

Lu-Jia Chen et al. Dis Markers. 2017.

Abstract

Background: Dysregulation of microRNAs may contribute to the progression of trauma-induced coagulopathy (TIC). We aimed to explore the biological function that miRNA-24-3p (miR-24) might have in coagulation factor deficiency after major trauma and TIC.

Methods: 15 healthy volunteers and 36 severe trauma patients (Injury Severity Score ≥ 16 were enrolled. TIC was determined as the initial international normalized ratio >1.5. The miR-24 expression and concentrations of factor X (FX) and factor XII in plasma were measured. In vitro study was conducted on L02 cell line.

Results: The plasma miR-24 expression was significantly elevated by 3.17-fold (P = 0.043) in major trauma patients and reduced after 3 days (P < 0.01). The expression level was significantly higher in TIC than in non-TIC patients (P = 0.040). Multivariate analysis showed that the higher miR-24 expression was associated with TIC. The plasma concentration of FX in TIC patients was significantly lower than in the non-TIC ones (P = 0.030) and controls (P < 0.01). A negative correlation was observed between miR-24 and FX. miR-24 transduction significantly reduced the FX level in the supernatant of L02 cells (P = 0.030).

Conclusions: miR-24 was overexpressed in major trauma and TIC patients. The negative correlation of miR-24 with FX suggested the possibility that miR-24 might inhibit the synthesis of FX during TIC.

PubMed Disclaimer

Figures

Figure 1
Figure 1
(a) Comparison of plasma expression levels of miR-24 in 36 major trauma patients and 15 controls. Plasma expression levels of miR-24 were higher in all major trauma patients than in healthy controls. ∗P < 0.05. (b) Plasma expression levels of miR-24 were significantly increased in 8 TIC patients than in the 28 non-TIC ones on ED arrival. MiR-24 expression levels were significantly downregulated on day 3 compared with those on day 0 (n = 24 in the non-TIC day-3 group and n = 7 TIC day 3 group). Compared to the non-TIC day 0 group, ∗P < 0.05, ∗∗P < 0.01, compared to the TIC day 0 group, #P < 0.01.
Figure 2
Figure 2
(a) The average level of FX in 8 TIC patients was significantly lower than that in 15 controls and 28 non-TIC patients. (b) Reduction in the average level of FXII in TIC patients compared to that in healthy controls was also noted. ∗P < 0.05, ∗∗P < 0.01.
Figure 3
Figure 3
Correlation analysis for miR-24 expression and the level of FX (n = 36).
See this image and copyright information in PMC

References

    1. Heim C., Bosisio F., Roth A., et al. Is trauma in Switzerland any different? Epidemiology and patterns of injury in major trauma - a 5-year review from a Swiss trauma centre. Swiss Medical Weekly. 2014;144:p. w13958. doi: 10.4414/smw.2014.13958. - DOI - PubMed
    1. Florence C., Simon T., Haegerich T., Luo F., Zhou C. Estimated lifetime medical and work-loss costs of fatal injuries--United States, 2013. MMWR. Morbidity and Mortality Weekly Report. 2015;64(38):1074–1077. doi: 10.15585/mmwr.mm6438a4. - DOI - PubMed
    1. Maegele M., Spinella P. C., Schochl H. The acute coagulopathy of trauma: mechanisms and tools for risk stratification. Shock. 2012;38(5):450–458. doi: 10.1097/SHK.0b013e31826dbd23. - DOI - PubMed
    1. Scott R., Jr., Crosby W. H. Changes in the coagulation mechanism following wounding and resuscitation with stored blood; a study of battle casualties in Korea. Blood. 1954;9(6):609–621. - PubMed
    1. Harr J. N., Moore E. E., Wohlauer M. V., et al. The acute coagulopathy of trauma is due to impaired initial thrombin generation but not clot formation or clot strength. The Journal of Surgical Research. 2011;170(2):319–324. doi: 10.1016/j.jss.2011.03.047. - DOI - PMC - PubMed

MeSH terms