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Case Reports
. 2017 Jul 6:12:36.
doi: 10.1186/s40793-017-0248-0. eCollection 2017.

Complete genome sequence of Microbulbifer sp. CCB-MM1, a halophile isolated from Matang Mangrove Forest, Malaysia

Tsu Horng Moh  1 , Nyok-Sean Lau  1 , Go Furusawa  1 , Al-Ashraf Abdullah Amirul  1   2
Affiliations
Case Reports

Complete genome sequence of Microbulbifer sp. CCB-MM1, a halophile isolated from Matang Mangrove Forest, Malaysia

Tsu Horng Moh et al. Stand Genomic Sci. .

Abstract

Microbulbifer sp. CCB-MM1 is a halophile isolated from estuarine sediment of Matang Mangrove Forest, Malaysia. Based on 16S rRNA gene sequence analysis, strain CCB-MM1 is a potentially new species of genus Microbulbifer. Here we describe its features and present its complete genome sequence with annotation. The genome sequence is 3.86 Mb in size with GC content of 58.85%, harbouring 3313 protein coding genes and 92 RNA genes. A total of 71 genes associated with carbohydrate active enzymes were found using dbCAN. Ectoine biosynthetic genes, ectABC operon and ask_ect were detected using antiSMASH 3.0. Cell shape determination genes, mreBCD operon, rodA and rodZ were annotated, congruent with the rod-coccus cell cycle of the strain CCB-MM1. In addition, putative mreBCD operon regulatory gene, bolA was detected, which might be associated with the regulation of rod-coccus cell cycle observed from the strain.

Keywords: Complete genome sequence; Estuarine sediment; Halophile; Mangrove; Microbulbifer.

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Conflict of interest statement

Competing interests

The authors declare that they have no competing interests.

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Figures

Fig. 1
Fig. 1
Scanning electron micrograph of Microbulbifer sp. CCB-MM1 at (a) exponential and (b) stationary phase
Fig. 2
Fig. 2
Neighbor-joining phylogenetic tree highlighting the position of Microbulbifer sp. CCB-MM1 relative to other type strains within the genus Microbulbifer, built using MEGA6 based on 16S rRNA sequences with their GenBank accession numbers indicated in parentheses
Fig. 3
Fig. 3
Circular map of the genome of Microbulbifer sp. CCB-MM1 generated using CGView Comparison Tool [44]. Circles (from outside) representing the following: 1. COG functional categories for forward coding sequence; 2. Forward sequence features; 3. Reverse sequence features; 4. COG functional categories for reverse coding sequence; 5. GC content; 6. GC skew
Fig. 4
Fig. 4
Circular map comparing strain CCB-MM1 genome and seven other Microbulbifer genomes generated using CGView Comparison Tool [44]. The two outermost rings represent forward and reverse sequence features respectively. The remaining seven rings show the regions of sequence similarity detected by BLAST comparisons conducted between nucleotide sequences from the CCB-MM1 genome and seven other Microbulbifer genomes with the order (from outside) as follow: Microbulbifer elongatus HZ11, Microbulbifer sp. Q7, Microbulbifer sp. WRN-8, Microbulbifer sp. ZGT114, Microbulbifer agarilyticus S89, Microbulbifer thermotolerans DAU221 and Microbulbifer variabilis ATCC 700307T
See this image and copyright information in PMC

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