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. 2018 Jan;11(1):112-118.
doi: 10.1111/1751-7915.12760. Epub 2017 Jul 11.

The bioelectric well: a novel approach for in situ treatment of hydrocarbon-contaminated groundwater

Enza Palma  1   2 , Matteo Daghio  3 , Andrea Franzetti  3 , Marco Petrangeli Papini  1 , Federico Aulenta  2
Affiliations

The bioelectric well: a novel approach for in situ treatment of hydrocarbon-contaminated groundwater

Enza Palma et al. Microb Biotechnol. 2018 Jan.

Abstract

Groundwater contamination by petroleum hydrocarbons (PHs) is a widespread problem which poses serious environmental and health concerns. Recently, microbial electrochemical technologies (MET) have attracted considerable attention for remediation applications, having the potential to overcome some of the limiting factors of conventional in situ bioremediation systems. So far, field-scale application of MET has been largely hindered by the limited availability of scalable system configurations. Here, we describe the 'bioelectric well' a bioelectrochemical reactor configuration, which can be installed directly within groundwater wells and can be applied for in situ treatment of organic contaminants, such as PHs. A laboratory-scale prototype of the bioelectric well has been set up and operated in continuous-flow regime with phenol as the model contaminant. The best performance was obtained when the system was inoculated with refinery sludge and the anode potentiostatically controlled at +0.2 V versus SHE. Under this condition, the influent phenol (25 mg l-1 ) was nearly completely (99.5 ± 0.4%) removed, with an average degradation rate of 59 ± 3 mg l-1 d and a coulombic efficiency of 104 ± 4%. Microbial community analysis revealed a remarkable enrichment of Geobacter species on the surface of the graphite anode, clearly pointing to a direct involvement of this electro-active bacterium in the current-generating and phenol-oxidizing process.

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Figures

Figure 1
Figure 1
Performance of the bioelectric well throughout the experimental period.
Figure 2
Figure 2
Taxonomic composition of the bacterial communities at the genus level. Only the genera with abundance of 5%, or higher, in at least one sample have been reported.
Figure 3
Figure 3
Cross‐sectional (A) and plan (B) view of an in situ groundwater bioremediation system based on the bioelectric well concept.
Figure 4
Figure 4
A. Schematic drawing of the laboratory‐scale scale experimental set‐up. B. Cross‐sectional view of the bioelectric well.
See this image and copyright information in PMC

References

    1. Atlas, R.M. (1995) Bioremediation of petroleum pollutants. Int Biodeterior Biodegradation 35: 317–327.
    1. Aulenta, F. , Canosa, A. , Reale, P. , Rossetti, S. , Panero, S. , and Majone, M. (2009) Microbial reductive dechlorination of trichloroethene to ethene with electrodes serving as electron donors without the external addition of redox mediators. Biotechnol Bioeng 103: 85–91. - PubMed
    1. Balch, W.E. , Fox, G.E. , Magrum, L.J. , Woese, C.R. , and Wolfe, R.S. (1979) Methanogens: reevaluation of a unique biological group. Microbiol Rev 43: 260–296. - PMC - PubMed
    1. Boopathy, R. (2000) Factors limiting bioremediation technologies. Bioresour Technol 74: 63–67.
    1. Daghio, M. , Vaiopoulou, E. , Patil, S.A. , Suárez‐Suárez, A. , Head, I.M. , Franzetti, A. , and Rabaey, K. (2016) Anodes stimulate anaerobic toluene degradation via sulfur cycling in marine sediments. Appl Environ Microbiol 82: 297–307. - PMC - PubMed

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