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. 1979 Apr;76(4):1585-8.
doi: 10.1073/pnas.76.4.1585.

Isolation of maxicircle component of kinetoplast DNA from hemoflagellate protozoa

L Simpson

Isolation of maxicircle component of kinetoplast DNA from hemoflagellate protozoa

L Simpson. Proc Natl Acad Sci U S A. 1979 Apr.

Abstract

Maxicircle DNA has been isolated from the kinetoplast DNA of Leishmania tarentolae culture forms by bouyant separation in CsCl in the presence of the A + T-binding dye Hoechst 33258, after liberation from the kinetoplast DNA network by cleavage with the single-hit restriction endonuclease EcoRI. The purified linearized maxicircle DNA has a density in CsCl of 1.681 g/cm3 (79% A + T) and a molecular weight of approximately 18-20 x 10(6). The maxicircle molecule exhibited intramolecular base composition heterogeneity ranging from 85% A + T to 74% A + T.

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References

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