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. 1979 May;76(5):2232-5.
doi: 10.1073/pnas.76.5.2232.

Structure-independent nucleotide sequence analysis

D R Mills, F R Kramer

Structure-independent nucleotide sequence analysis

D R Mills et al. Proc Natl Acad Sci U S A. 1979 May.

Abstract

Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.

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References

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