doi: 10.1016/j.mimet.2017.07.002.
Epub 2017 Jul 12.
Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus-related and V. aestuarianus strains from bacterial cultures
Affiliations
- PMID: 28709898
- DOI: 10.1016/j.mimet.2017.07.002
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Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus-related and V. aestuarianus strains from bacterial cultures
J Microbiol Methods.
2017 Sep.
Abstract
To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies.
Keywords: Gene sequencing; Molluscs; Multiplex real-time PCR; Splendidus clade; Vibrio aestuarianus.
Copyright © 2017 Elsevier B.V. All rights reserved.
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