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. 2017 Sep:140:67-69.
doi: 10.1016/j.mimet.2017.07.002. Epub 2017 Jul 12.

Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus-related and V. aestuarianus strains from bacterial cultures

Denis Saulnier  1 , Sophie De Decker  2 , Delphine Tourbiez  2 , Marie Agnès Travers  2
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Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus-related and V. aestuarianus strains from bacterial cultures

Denis Saulnier et al. J Microbiol Methods. 2017 Sep.

Abstract

To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies.

Keywords: Gene sequencing; Molluscs; Multiplex real-time PCR; Splendidus clade; Vibrio aestuarianus.

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