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Comment
. 2017 Aug 7;216(8):2239-2241.
doi: 10.1083/jcb.201706005. Epub 2017 Jul 17.

Strength in numbers: Phosphofructokinase polymerization prevails in the liver

Elma Zaganjor  1 , Jessica B Spinelli  1 , Marcia C Haigis  2
Affiliations
Comment

Strength in numbers: Phosphofructokinase polymerization prevails in the liver

Elma Zaganjor et al. J Cell Biol. .

Abstract

Numerous metabolic enzymes assemble into filamentous structures, which are thought to serve additional regulatory functions. In this issue, Webb et al. (2017. J. Cell Biol. https://doi.org/10.1083/jcb.201701084) show that the liver-specific isoform of phosphofructokinase-1 forms filaments in vitro and localizes as puncta in cells along the plasma membrane. This suggests spatial organization of glycolysis in higher organisms.

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Figures

Figure 1.
Figure 1.
The liver isoform of PFKL, a gatekeeper of glycolysis, forms filaments. (A) Schematic of glucose usage. Glucose is broken down and used for numerous metabolic processes, including for ATP-generating glycolysis. PFK1 is an irreversible regulator and thus commits glucose to oxidative degradation. (B) PFK1 has two states of quaternary structure: the inhibitory T state and the activating R state. The T state is regulated by ATP, citrate, and glycosylation; the R state is stabilized by AMP and F2,6BP, causing increased glycolytic flux. The liver isoform of PFK1 is further regulated through filament formation.
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